Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Vilnius University

Stephane Deschamps

Joshua K Young

Zhongsen Li

James J English

Virginijus Siksnys

Mary Beatty

Gina Marie Zastrow-Hayes

Victor Llaca

Gregory D May

Nicholas Doane Chilcoat

Mindaugas Zaremba

Methods and compositions are provided for the identification, detection, characterization, and/or utilization of double strand breaks in a target polynucleotide; the identification, detection, characterization, and/or utilization of cutting sites for double-strand-break-inducing agents; and the identification, detection, characterization, and/or utilization of double-strand-break-inducing agents.

Methods for the identification and characterization of double-strand break sites and compositions and uses thereof

Compositions and methods of identifying and characterizing potential gene editing on-target and off-target sites and/or edits in a nucleic acid are provided.

Methods of identifying and characterizing gene editing variations in nucleic acids

Methods for the identification of variant recognition sites for rare cutting engineered double strand break inducing agents and compositions thereof are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the of variant recognition sites. Various methods of identifying variant recognition sites with increased substrate activity for a rare cutting engineered double strand break inducing agents are provided.

Methods for the identification of variant recognition sites for rare-cutting engineered double-strand-break-inducing agents and compositions and uses thereof

Growing community of inventors

Hockessin, DE, United States of America

Stephane Deschamps