10x Genomics, Inc.

Stephane Claude Boutet

Katherine Pfeiffer

Michael Ybarra Lucero

Tarjei Sigurd Mikkelsen

Phillip Belgrader

Serge Saxonov

Rajiv Bharadwaj

Christopher Hindson

John R Stuelpnagel

Andrew D Price

Geoffrey McDermott

Tobias Daniel Wheeler

Zachary Bent

Paul Ryvkin

Jessica Michele Terry

Mohammad Rahimi Lenji

Josephine Harada

Elliott Meer

Indira Wu

Niranjan Srinivas

Vijay Kumar Sreenivasa Gopalan

Solongo B Ziraldo

Sarah Taylor

Christopher Joachim O'Keeffe

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for 'tagging' analytes for characterization.

Methods and systems for processing polynucleotides

A method of analyzing single biological particles, such as cells or nuclei, that maintains the single biological particles in a state of relative isolation during decrosslinking and subsequent processing is provided. In the case of cells, the method prevents cellular analytes from each individual cell from leaving the cell site and diffusing toward adjacent cells, while permitting transmission of a decrosslinking agent, followed by processing of the cellular analytes from the individual cells by barcoding and/or imaging of the cellular analytes.

Solid state single cell method for analyzing fixed biological cells

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Burlingame, CA, United States of America

Stephane Claude Boutet