Hoffmann-la Roche Inc.

Stephan Doswald

Ernst Kupfer

Gerhard Steinbauer

Steven Paul Hanlon

Erich Steinwender

The invention relates to a scalable biocatalytic process for the preparation of S-1,1,1-trifluoro-2-propanol with a enantiomeric excess of &#x3e;99% by asymmetric microbial reduction of 1,1,1-trifluoroacetone with Baker's yeast.

Asymmetric reduction of 1,1,1-trifluoroacetone

The present invention relates to a method for the purification of the lipstatin. Impurities are removed from a lipstatin preparation by: a) mixing the preparation with a polar phase and to form a first two phase system in which the ratio of the lipstatin to the non-polar impurities in the polar phase is greater than in the preparation, and separating the phases from the first two phase system and collecting the lipstatin-enriched polar phase, and b) i) diluting the lipstatin-enriched polar phase from step a) with water to form a diluted lipstatin-enriched polar phase containing the polar solvent in a percentage of at least seventy percent of the polar phase and lower than in the lipstatin-enriched polar phase from step a), and mixing the diluted lipstatin-enriched polar phase with a second non-polar phase, the second non-polar phase being the same non-polar solvent as the first non-polar phase in the same or greater relative amount to the polar phase as in the first two phase system; or ii) mixing the lipstatin-enriched polar phase from step a) with a second non-polar phase, the second non-polar phase being the same non-polar solvent as the first non-polar phase in a greater relative amount to the polar phase as in the first two phase system; to form a second two phase system in which the ratio of the lipstatin to the polar impurities in the second non-polar phase is greater than in the lipid-enriched polar phase from step a), and separating the phases from the second two phase system and collecting the lipstatin-enriched second non-polar phase.

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Reinach, Switzerland

Stephan Doswald