Forschungszentrum Julich Gmbh

Forschungszentrum Juelich Gmbh

Senseup Gmbh

Stephan Binder

Lothar Eggeling

Michael Bott

Georg Schendzielorz

Stephanie Bringer-Meyer

Solvej Siedler

Sarah-Kristin Jurischka

Georg Schaumann

Britta Kleine

Roland Freudl

Julia Frunzke

Nurije Mustafi

An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Sensor for NADP (H) and development of alcohol dehydrogenases

The present invention relates to a cell which is genetically modified with respect to its wild type and which comprises a gene sequence coding for a fluorescent protein, wherein the expression of the fluorescent protein depends on the amount of protein that is secreted across the cytoplasmic membrane into the extracytosolic space. The present invention also relates to a method for the identification of a cell having an increased secretion of protein across the cytoplasmic membrane into the extracytosolic space, a method for the identification of a culture medium composition that is optimized for the recombinant production of protein, a method for the identification of culture conditions that are optimized for the recombinant production of protein, a method for the identification of a compound that is characterized by an antibiotic activity due to its property to damage the membrane of a bacterial cell or to analyse the effect of such a compound on a population of genetically different bacterial cells or genetically identical cells in different physiological states or different growths phases, a method for the production of a cell which is genetically modified with respect to its wild type with optimized secretion of protein across the cytoplasmic membrane into the extracytosolic space, a cell obtained by this method, a method for the production of proteins and a method for the preparation of a mixture.

Sensors for detection and quantification of microbiological protein secretion

The present invention relates to an NADP(H) nanosensor comprising The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

A method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and to the use thereof for producing amino acids and nucleotide. Genes coding for feedback-inhibited enzymes are mutagenized in vitro, &#x2014;ligated into vectors in a further step&#x2014;the vectors are each transformed in a microorganism containing a metabolite sensor in a further step, which brings about the synthesis of a fluorescent protein at an increased metabolite concentration, &#x2014;whereupon microorganisms exhibiting increased or maximum fluorescence are selected; and &#x2014;the vectors that contain a gene coding for an enzyme having reduced or deactivated feedback inhibition are isolated from microorganisms having increased or maximum fluorescence.

Method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and the use thereof for producing amino acids and nucleotides

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Eschweiler, Germany

Stephan Binder