Evonik Operations Gmbh

Steffen Oßwald

Jakob Müller

Markus Pötter

Ludger Lautenschütz

Christian Bangert

An enzymatic method for the production of an L-glufosinate P-alkyl ester can be performed. This method is characterized by reacting an L-glufosinate P-alkyl ester carbamoylate to give the corresponding L-glufosinate P-alkyl ester. This is catalyzed by a carbamoylase. The L-glufosinate P-alkyl ester carbamoylate employed may be obtained by a reaction from the corresponding L-glufosinate hydantoin P-alkyl ester. This is catalyzed by a hydantoinase, preferably an L-enantioselective hydantoinase. In a second aspect, an enzymatic method for enantioselective production of an L-glufosinate P-alkyl ester from a mixture Mof L- and D-glufosinate P-alkyl ester hydantoins can be performed. The L-glufosinate P-alkyl ester obtained in the methods according to the first or second aspect of the invention may be saponified to give L-glufosinate.

Enzymatic method for the production of l-glufosinate p-alkyl esters

A first enzymatic method for the production of an L-glufosinate P-ester includes reacting an L-glufosinate P-ester carbamoylate to give the corresponding L-glufosinate P-ester in the presence of a carbamoylase as a catalyzer. A second enzymatic method includes the enantioselective production of an L-glufosinate P-ester from a mixture Mof L- and D-glufosinate P-ester hydantoins. The L-glufosinate P-ester obtained in the two methods may be saponified to give L-glufosinate.

Enzymatic method for the production of L-glufosinate P-esters

A method for producing a recombinant collagen-like protein (CLP) can be performed. The method includes fermenting a host cell, accumulating the CLP in a medium to obtain a fermentation broth, separating the host cell from the fermentation broth to obtain a supernatant, and incubating the supernatant. The CLP can be purified after incubation.

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Nidderau, Germany

Steffen Oßwald