Evonik Degussa Gmbh

Degussa Aktiengesellschaft

Steffen Osswald

Klaus Huthmacher

Christoph Weckbecker

Maria-Regina Kula

Michael Binder

Stefan Verseck

Tatijana Gerasimova

Andrey Novikov

Ludmila Ryabchenko

Alexander Yanenko

Ksenia Egorova

Uta Deiting

Konrad Odendahl

Werner Hummel

Birgit Geueke

Disclosed herein are cyanide-tolerant nitrile hydratases especially fromorstrains which exhibit increased cyanide tolerance. Also disclosed are methods of preparing amides from nitriles in the presence of cyanides and polynucleotide sequences coding for cyanide-tolerant nitrile hydratases.

Polynucleotides which code for cyanide-tolerant nitrile hydratase and compositions thereof

Process for the enzymatic preparation of an amide from a nitrile

The invention relates to apolynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein PK which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.

Rhodococcus nitrile hydratase

The invention relates to apolynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.

The invention relates to recombinant microorganisms which, in comparison to the starting organism, have a higher concentration or activity of a D-amino acid oxidase, of an L-amino acid dehydrogenase, of an NADH cosubstrate regenerating enzyme and, if necessary, of a catalase. The invention also includes methods for preparing L-amino acids from D-amino acids using of these microorganisms.

Method for the preparation of L-amino acids from D-amino acids

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Rodenbach, Germany

Steffen Osswald