Zyomyx, Incorporated

Zyomix, Inc.

Steffen Nock

Peter K Wagner

Christian Itin

Dana Ault-Riche

Frank G Zaugg

Pierre Indermuhle

Paul Jedrzejewski

David Wilson

Paul D Kassner

Jens Sydor

Jiangchun Wu

Lifu Ma

Embodiments of the invention are directed to microfluidic devices. In one embodiment, a microanalysis chip comprises a body having at least one transfer-separation channel with a channel bottom that has a bottom opening. The transfer-separation channel terminates in a discharge aperture.

Microfluidic devices and methods

Heterofunctional crosslinking groups are provided having the formula: wherein W is a covalent core component; L, Land Lare independently linking groups; X is a non-covalent or reversibly covalent protein tag binder; Y is a activatable covalent linking group; and Z is a protected or unprotected covalent crosslinking group.

Site-specific, covalent bioconjugation of proteins

Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.

Arrays of protein-capture agents and methods of use thereof

A chip is disclosed. The chip includes a base including a non-sample surface and at least one structure includes a pillar. Each structure includes a sample surface that is elevated with respect to the non-sample surface and each structure may be adapted to receive a sample.

Chips having elevated sample surfaces

Improved methods for making antibody fragments, preferably F(ab&prime;) fragments from various classes and subclasses of antibodies is described. Pretreatment of antibodies with deglycosylases or cellular inhibition of glycosylation during expression, yields antibodies having improved susceptibility towards protease cleavage, preferably pepsinolysis, which yields F(ab&prime;) antibody fragments. Compositions resulting from such methods are further disclosed.

Methods for making antibody fragments and compositions resulting therefrom

This invention provides methods for screening populations of phage-displayed polypeptides that are particularly well-suited for high-throughput screening. The methods do not require the clearing of cells from a culture used to obtain the population of phage or other replicable genetic packages. Accordingly, the invention provides methods for forming complexes between a replicable genetic package displaying a polypeptide fusion and a target molecule in an uncleared cell culture containing replicable genetic package. Compositions made up of an uncleared cell culture containing replicable genetic packages displaying a polypeptide fusion and a target molecule are provided in the invention as well.

Screening of phage displayed peptides without clearing of the cell culture

Methods for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules immobilized on the surface of the devices employed in the method of the present invention include proteins, polypeptides, polynucleotides, polysaccharides, phospholipids, and related unnatural polymers of biological relevance. These methods are useful in drug development, functional proteomics studies and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.

Microdevices for screening biomolecules

Protein arrays for the parallel, in vitro screening of biomolecular activity are provided. Methods of using the protein arrays are also disclosed. On the arrays, a plurality of different proteins, such as different members of a single protein family, are immobilized on one or more organic thinfilms on the substrate surface. The protein arrays are particularly useful in drug development, proteomics, and clinical diagnostics.

Arrays of proteins and methods of use thereof

Devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules immobilized on the surface of the devices of the present invention include proteins, polypeptides, polynucleotides, polysaccharides, phospolipids, and related unnatural polymers of biological relevance. These devices are useful drug development, functional proteomics and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.

Methods for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules that can be immobilized on the surface of the devices of the present invention include proteins, polypeptides, nucleic acids, polysaccharides, phospholipids, and related unnatural plyomers of biological relevance. These devices are useful in high-throughput drug screening and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.

Microdevices for high-throughput screening of biomolecules

Devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules that can be immobilized on the surface of the devices of the present invention include proteins, polypeptides, nucleic acids, polysaccharides, phospolipids, and related unnatural polymers of biological relevance. These devices are useful in high-throughput drug screening and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.

Protein arrays and protein-coated substrates for the parallel, in vitro screening of biomolecular activity are provided. Methods of using the protein-coated substrates and protein arrays are also disclosed. A plurality of different members of a single protein family may be immobilized on the protein-coated substrate or array. The protein-coated substrates and protein arrays are particularly useful in high-throughput drug screening and clinical diagnostics.

Growing community of inventors

Redwood City, CA, United States of America

Steffen Nock