Technische Universitat Darmstadt

Istitut Fur Mikrotechnik Mainz Gmbh

Steffen Hardt

Ulrike Nuber

Heinz Koeppl

Katrin Töpfer

A fluidic device, a fluidic system and a method for developing a cellular starting material into a three-dimensional cellular structure. The fluidic device includes a base body which includes a chamber in which a matrix is received, into which the cellular starting material to be developed can be introduced, and at least two fluid reservoirs. Each fluid reservoir includes a fluid inlet, a fluid outlet and a separating device which is partially permeable to a fluid medium and which separates the associated fluid reservoir from the chamber and forms a common plane interface with the chamber, via which the fluid medium can diffuse into the matrix. When using suitable fluid media, the fluidic device is adapted to form at least one concentration gradient, at least two mutually orthogonal concentration gradients and/or at least two mutually antiparallel concentration gradients in the matrix, each of which are essentially homogeneous or deliberately inhomogeneous in the z-direction over at least a section of the extension of the matrix.

Fluidic device, fluidic system, and method for developing three-dimensional cellular constructions

Electric fields are applied parallel to and/or vertical to the interfaces on adjacent microfluid lamellae made of nonmiscible media containing said biomolecules and bioparticles to which they have different physico-chemical affinities in order to separate biomolecules and bioparticles and the biomolecules and bioparticles are electrophoretically separated.

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Darmstadt, Germany

Steffen Hardt