Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Kautex Textron Gmbh & Co. Kg

Nanotag Biotechnologies Gmbh

Steffen Frey

Dirk Goerlich

Dirk Gorlich

Matthias Borchert

Carsten Elsasser

Christoph Mehren

Thomas Buchholz

Jochen Hild

Deniz Bienhuls

Marcus Schmitz

Hansjoerg Goetzke

Davilla Luis Felipe Opazo

Paal Erik Gustav Stenmark

Carranza Markel Martinez

Arturo Vera Rodriguez

Dirk Görlich

Felipe Opazo

Hansjörg GÖTZKE

Deniz Bienhüls

Deniz Bienhuels

The present invention provides peptides useful as epitope tags, which may be fused to a polypeptide of interest, as well as antibodies that specifically bind to these peptides. The peptides and/or antibodies can be used for detecting, immobilizing, isolating or purifying a molecule that is conjugated to such a peptide and/or antibody.

Epitope tags recognized by specific binders

The present invention relates to a fusion protein, comprising the structure N-PCS-degSig-M-PCS-degSig-C; wherein N represents the N-terminus; PCSand PCSeach represent a protease cleavage site (PCS), which differ from each other in at least one amino acid residue: degSigrepresents a degradation signal which promotes degradation of the fusion protein in a host cell if PCSis cleaved by a protease such that the first amino acid of degSigbecomes the new N-terminus of the remaining fusion; M represents a cytoplasmic selection marker; and degSigrepresents a second degradation signal which promotes degradation of the fusion protein in a host cell if PCSis not cleaved by a protease; and C represents the C-terminus. Further provided is a nucleic acid construct, comprising a nucleic acid sequence coding for said fusion protein, a nucleic acid expression construct library, comprising a plurality of such nucleic acid expression constructs in diversified form, and methods using the fusion protein and nucleic acid constructs coding therefor. Finally, the present invention provides variants of bdSUMO and bdSENP1 which have been identified by the methods of the present disclosure, and which exhibit improved properties over existing orthogonal protease/protease cleavage site-pairs which are currently used with wild-type bdSUMO and wildtype bdSENP1.

Protease specific for a novel sumo-derived protease cleavage site

The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.

Immobilisation of chelating groups for immobilised metal ion chromatography (IMAC)

The present invention belongs to the field of biotechnology. More specifically, the present invention provides a protease, a non-naturally occurring fusion protein comprising a corresponding protease recognition site, expression vectors encoding same, host cells comprising said expression vectors, kit of parts as well as methods applying the protease, fusion protein, and uses thereof, as defined in the claims. The presently disclosed protease/protease recognition site is particularly useful in methods requiring an orthogonal set of proteases, and is suitable for use in both prokaryotic and selected eukaryotic expression systems.

Tag removal from proteins expressed in pro- and eukaryotic hosts

Immobilization of chelating groups for immobilized metal ion chromatography (IMAC)

A blow molding tool () for producing a plastic article, in particular a fuel tank, comprising at least one clamping frame () with mold clamping devices () and at least two blow mold halves, which are formed such that they can be moved together and apart by means of an opening and closing movement of the clamping frame () and which respectively have at least one cavity () for the forming of the plastic article, the blow molding tool () being distinguished by the fact that the mold clamping devices () respectively have at least one tool holder (), in which a mold insert () is in each case exchangeably inserted, and in that the tool holders () respectively form a peripheral sealing edge ().

Blow molding tool and method for producing extrusion blow molded plastic articles

Growing community of inventors

Goettingen-Herberhausen, Germany

Steffen Frey