Centre National De La Recherche Scientifique

Valorisation-recherche, Limited Partnership

Other

Institut National De La Sante Et De La Recherche Medicale

Université Paris Descartes

Stefano Marullo

Xavier Nassif

Laurent Jean Emorine

Donny Strosberg

Michel Bouvier

Arthur Donny Strosberg

Thomas P Loisel

Pierre Boulanger

Colette Delavier

Mathieu Coureuil

The present invention describes a method for producing membrane receptors based on a baculovirus/insect cell system. The invention further includes the receptors produced by the method described herein, a model for studying the properties of these membrane receptors and an assay to screen molecules that are active on these membrane receptors.

Preparing membrane receptors from extracellular baculoviruses

Method of producing a recombinant membrane receptor protein by introducing a baculovirus expression vector encoding the membrane receptor protein into a host cell, culturing the resultant host cell for a sufficient time to permit expression of the membrane receptor protein and baculovirus viral particles and separating the cells from the viral particles. The baculovirus viral particles are then recovered.

Preparing membrane receptors from extracellular baciloviruses

The present invention relates to the intron and exon structure of the gene encoding &#x3b2;3 adrenergic receptor polypeptides of mouse and human origin, which polypeptides are useful in a procedure for studying the effects of various chemical agents on the &#x3b2;3 adrenergic receptor coupled to adenylate cyclase and hormone-sensitive lipases.

Intron/exon structure of the human and mouse &#x3b2;3-adrenergic receptor genes

Method for producing membrane receptors based on a baculovirus/insect cell system. The invention further includes the receptors produced by the method described herein, a model for studying the properties of these membrane receptors and an assay to screen molecules that are active on these membrane receptors.

The invention relates to a vector capable of being replicated in cultures of unicellular organisms, this vector containing a gene coding for a eukaryotic protein having the biological activity of a membrane receptor and interacting with a regulatory protein&mdash;called the G protein&mdash;able to bind molecules of guanosine triphosphate (GTP). The invention also relates to cell organisms transformed by the above vectors. It also relates to procedures for the detection of the capacity of a molecule to behave as a ligand for a receptor and a procedure for studying the affinity of a receptor for a ligand as well as a kit for detecting the possible affinity of a ligand for a receptor.

VECTOR MODIFIED BY SEQUENCE CODING FOR A SEQUENCE OF AMINO ACIDS CONTAINED IN A MAMMALIAN PROTEIN HAVING THE BIOLOGICAL ACTIVITY OF A MEMBRANE RECEPTOR, EXPRESSION OF THIS SEQUENCE IN TRANSFORMED UNICELLULAR CULTURES BY MEANS OF THIS VECTOR, PROCEDURE FOR STUDYING LIGANDS RECOGNIZING THESE RECEPTORS

The present invention relates to the intron and exon structure of the gene encoding &bgr;3 adrenergic receptor polypeptides of mouse and human origin, which polypeptides are useful in a procedure for studying the effects of various chemical agents on the &bgr;3 adrenergic receptor coupled to adenylate cyclase and hormone-sensitive lipases.

Intron/exon structure of the human and mouse &bgr;3-adrenergic receptor genes

Intron/exon of the human and mouse beta 3-adrenergic receptor genes

Polypeptides having a .beta.-adrenergic receptor activity in man,

The invention relates to a vector capable of being replicated in cultures of unicellular organisms, this vector containing a gene coding for a eucaryotic protein having the biological activity of a membrane receptor and interacting with a regulatory protein--called the G protein--able to bind molecules of guanosine triphosphate (GTP). The invention also relates to cell organisms transformed by the above vectors. It also relates to procedures for the detection of the capacity of a molecule to behave as a ligand for a receptor and a procedure for studying the affinity of a receptor for a ligand as well as a kit for detecting the possible affinity of a ligand for a receptor.

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Paris, France

Stefano Marullo