Morphosys Ag

Stefanie Thiel

Josef Prassler

Hilmar Erhard Ebersbach

Thomas Pietzonka

Yvonne Stark

David Ott

Ute Keck

Christine Rothe

Stefanie Urlinger

Markus Enzelberger

Urlinger Stefanie

Prassler Josef

Rothe Christine

The present invention provides synthetic rodent antibody libraries, such as mouse or rat antibody libraries, as well as polypeptides, nucleic acids, vectors, host cells and methods used in conjunction with these libraries. The present invention also provides antibodies isolated from such libraries and variants of such antibodies.

Rodent combinatorial antibody libraries

The present invention is directed to the preparation and use of a collection of antibody heavy chain complementarity determining region 3 (HCDR) members, where diversity of the collection is a function of the length of the HCDRmembers. The diversity of the collection of HCDRregions substantially represents the natural amino acid distribution of HCDRin the human repertoire. This natural amino acid distribution can be represented by biasing the complete random distribution of amino acids, accordingly, in the HCDRencoding DNA sequence by using trinucleotide mutagenesis (TRIM) technology. A collection of HCDRmembers of the invention each can be comprised within a variable region of an antibody (or fragment thereof) to form a library of synthetic antibodies or antibody fragments. The invention also provides nucleic acid molecules encoding such diverse collection and methods of making and using the same.

Collection of HCDR3 regions and uses therefor

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Munich, Germany

Stefanie Thiel