Leland Stanford Junior University

Stefanie Rothkoetter

Nicholas A Melosh

Yuhong Cao

Sergio Leal-Ortiz

Ryan T Swoboda

Sergio Leal-ortiz

Described herein are nanostraw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane from a fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., &#x3e;70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.

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Stanford, CA, United States of America

Stefanie Rothkoetter