Regeneron Pharmaceuticals, Inc.

Regeneran Pharmaceuticals, Inc.

Stefanie McDermott

John Mattila

Nathan L Mao

Bernhard Schilling

Scott Carver

Hanne Bak

Eric Shierly

James Reilly

Isabelle Livigni

Embodiments of the present disclosure are directed to methods for preparing a target polypeptide from a mixture including the target polypeptide. The method may include contacting the mixture to a hydrophobic interaction chromatography (HIC) apparatus including multiple chromatographic zones. The method may further include passing the target polypeptide through the outlets of at least a first zone and a second zone of the HIC apparatus. A residence time for the mixture including the target polypeptide in a first zone may be approximately the same as a residence time of one or more mobile phases in the second zone.

Systems and methods for preparing a polypeptide from a mixture

Embodiments of the present disclosure are directed to methods and systems for assessing integrity of chromatography columns, systems, and processes. The methods and systems can comprise one or more of extracting a block and signal combination for analysis, performing a transition analysis, performing one or more statistical process controls, and/or implementing in-process controls based on the statistical process controls.

Methods and systems for chromatography data analysis

Growing community of inventors

Sleepy Hollow, NY, United States of America

Stefanie McDermott