Degussa Aktiengesellschaft

Evonik Degussa Gmbh

B.r.a.i.n. Biotechnology Research and Information Networks Ag

Evonik Goldschmidt Corporation

B.r.a.i.n. Biotechnology Research and Information Network Ag

Stefan Verseck

Karlheinz Drauz

Klaus Huthmacher

Christoph Weckbecker

Uwe Dingerdissen

Maria-Regina Kula

Andreas Sebastian Bommarius

Michael Binder

Konrad Odendahl

Uta Deiting

Steffen Schaffer

Andreas Bommarius

Gerda Grund

Friedrich Georg Schmidt

Jürgen Eck

Steffen Osswald

Matthias Orschel

Werner Freitag

Klaus Liebeton

Peter Duerre

Ksenia Egorova

Heike Slusarczyk

Antje May

Ralf-Joerg Fischer

Hubert Johannes Bahl

Marion Ansorge-Schumacher

Simone Lederle

Lutz Krieg

Wilfried Schmidt

Friedrich-Georg Schmidt

Peter Dürre

Wai-Yee Phong

Ralf-Jörg Fischer

Harald Trauthwein

Garabed Antranikian

The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO, which is characterized in that the coenzyme A is not transferred in process step B to an acceptor molecule. In addition, process step B is surprisingly catalyzed by enzymes of the classes of acyl-CoA thioesterase, acyl-CoA synthetase or acyl-CoA thiokinase. A completely novel metabolic pathway is concerned, because the enzymatic hydrolysis of acetoacetyl-CoA without simultaneous transfer of CoA to a receptor molecule has never previously been described for any microbial enzyme.

Fermentative production of acetone from renewable resources by means of novel metabolic pathway

The present invention is concerned with the preparation of novel nitrile hydratases. These latter are preferably obtained from nonculturable organisms by means of a PCR-based screening, in metagenome DNA libraries, using special degenerate primers.

Nitrile hydratases from metagenome libraries

The invention relates to apolynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein PK which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.

Rhodococcus nitrile hydratase

The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.

Process for the production of amino acids

The invention relates to apolynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.

Process for the racemization of N-protected amino acids using racemase

The present invention relates to an amidase enzyme, nucleic acids encoding the amidase, as well as methods of employing the nucleic acids and/or amidase to produce, for example, enantiomericallyenriched compounds such as D-amino acids.

Amidase from variovorax

The invention relates to the use of the N-acetyl amino acid racemase from subspecies for the racemiszation of N-carbamoyl amino acids. This use permits the 100% preparation of optically pure amino acids starting from racemic hydantoins in an enzymatic overall process.

Acetyl amino acid racemase from amycolatopsis orientalis for racemizing carbamoyl amino acids

Process for the production of amino acids using racemase and acylase

The present invention is directed to an N-acetyl amino acid racemase (AAR) as well as the gene coding for it, to a plasmid, vector and microroganism containing the gene. A method of producing enantiomer-enriched amino acids, and derivatives thereof, is also provided.

N-acetyl amino acid racemase

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Hanau, Germany

Stefan Verseck