Basf Se Corporation

Stefan Seemayer

Michael Breuer

Doreen Schachtschabel

Xuqiu Tan

Tobias Joachim Zimmermann

Stefan Jenewein

Max Fabian Felle

Jochen Kutscher

Adrienne Huston Davenport

Diego Ghislieri

Tong Li

Hugo Urbina

Kristine W Chang

Tuan Tran

Described herein are methods for the production of ammonium acrylate or salts thereof from acrylonitrile using nitrilase as a catalyst.

Method for the production of acrylic acid or salts thereof

The present invention is directed to a producing a DNA methyltransferase in a recombinant host cell, wherein the DNA methyltransferase methylates DNA resulting in a DNA containing 5-methylcytosine within the recognition sequence GCNGC and wherein the DNA methyltransferase comprises less than (35) amino acid residues between amino acid residue (72) and amino acid residue (106) according to the numbering of SEQ ID NO: 33. Furthermore, the present invention is directed to the use of such DNA methyltransferase for the production of bacterial transformants comprising the steps of (a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide sequence encoding the DNA methyltransferase to produce a methylated DNA and (b) transferring the methylated DNA from the first bacterial host cell into a second bacterial host cell, wherein the second bacterial host cell comprises a restriction endonuclease able to degrade the DNA but unable to degrade the methylated DNA.

Method of transforming bacterial cells

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Ludwigshafen, Germany

Stefan Seemayer