Roche Diagnostics Operations, Inc.

C-lecta Gmbh

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Stefan Schoenert

Thomas Greiner-Stoeffele

Andreas Vogel

Birgit Brucher

Claudia Feller

Sebastian Bartsch

Sabrina Koepke

Mathias Salomo

Thomas Schultchen

The present disclosure provides the sequence of apreproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

Methods of recombinantly producing neutral protease originating from

Expression vector encoding aneutral protease

The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.

Enzyme products

The present disclosure provides the sequence of a Paenibacillus polymyxa preproenzyme which is the precursor of a neutral protease, expression thereof in a transformed host organism, and methods for production of the neutral protease, by recombinant means. Further, use of the recombinantly produced neutral protease is disclosed in the field of cell biology, particularly for the purpose of tissue dissociation. The disclosure also includes blends with other proteases. Further disclosed are nucleotide sequences encoding the neutral protease.

Recombinantly produced neutral protease originating from Paenibacillus polymyxa

A method for producing a nuclease of a gram negative bacterium or a nuclease preparation containing a nuclease of a gram negative bacterium including expression of the nuclease in a gram positive bacterium and subsequent secretion of the nuclease, as well as a nuclease or a nuclease preparation that can be obtained by this method.

Method for producing serratia marcescens nuclease using a bacillus expression host

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Leipzig, Germany

Stefan Schoenert