Ncardia B.v.

Stefan Robbert Braam

Ana Catarina Martins Grandela

Christine Lindsay Mummery

Leon Tertoolen

Fleur Stevenhagen

Karin Langenberg

The current disclosure relates to a culture medium, different methods to generate adult-like cardiomyocytes from pluripotent embryonic stem cells (ESC) and/or (induced) pluripotent stem cells (iPSC) using the medium, in particular from stem cells that differentiated into (foetal) cardiomyocytes, and to kits comprising the medium, or the medium together with differentiated (foetal) cardiomyocytes derived from pluripotent embryonic stem cells (ESC) and/or (induced) pluripotent stem cells (iPSC).

Cardiomyocytes derived from pluripotent mammalian stem cells

The present invention is in the field of pluripotent stem cells, more particularly cardiomyocytes derived from pluripotent stem cells. The present invention provides a novel method for differentiating human pluripotent stem cells into a population of cardiomyocytes having an atrial phenotype, and use of said atrial cardiomyocytes for screening of drugs, AF disease model, and others. The method of the invention is particularly useful to generate cardiomyocytes having a more developed or mature atrialphenotype and/or to generate higher yield of cardiomyocytes having an atrialphenotype.

In vivo method for differentiating human pluripotent stem cells into atrial cardiomyocytes

The current invention relates to a method of differentiation of human pluripotent stem cells into a human stem-cell derived population of cardiomyocytes. The method comprises the use of specific combination of steps and compounds to induce and/or promote differentiation. The method also comprises steps directed to further maturation of the cardiomyocytes obtained with the method of the invention. Also provided are kits for use in a method of differentiation as well as cell populations obtainable with the method disclosed.

In vitro method of differentiating a human pluripotent stem cell population into a cardiomyocyte cell population

This disclosure generally concerns the fields of cell biology and molecular biology. In particular the invention concerns the field of stem cell biology and maturation of stem cell-derived cardiomyocytes. Disclosed is a method for improving the maturity of stem-cell derived cardiomyocytes, in particular of the ventricular type, as can be witnessed by, for example, an improved upstroke velocity of the stem-cell derived cardiomyocytes.

Cardiomyocyte maturation

Culture medium composition for maturating cardiomyocytes derived from pluripotent mammalian stem cells

The current disclosure relates to technology for predicting cardiotoxicity of compounds. In vitro methods using human stem-cell derived cardiomyocytes cultured in specific media are disclosed, as well as kits for use in the method. In the method, the cells are treated with a test compound in combination with a mitochondrial toxicant.

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Leiden, Netherlands

Stefan Robbert Braam