Nanogen Recognomics Gmbh

Stefan Raddatz

Norbert Windhab

Markus Schweitzer

Christoph Brücher

Richard R Anderson

Stefan Kienle

Michael D Fiechtner

John R Havens

Thomas J Onofrey

Charles Greef

Daguang Wang

Jochen Müller-Ibeler

Marc Pignot

Eberhard Schneider

Jill M Orwick

Jochen Müller

Jill Orwick

Richard Anderson

This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.

Hydrazide building blocks and hydrazide modified biomolecules

The present invention relates to conjugates of synthetic binding units and nucleic acids. The present invention also relates to methods for sorting and immobilizing nucleic acids on support materials using such conjugates by specific molecular addressing of the nucleic acids mediated by the synthetic binding systems. Particularly, the present invention also relates to novel methods of utilizing conjugates of synthetic binding units and nucleic acids to in active electronic array systems to produce novel array constructs from the conjugates, and the use of such constructs in various nucleic acid assay formats. In addition, the present invention relates to various novel forms of such conjugates, improved methods of making solid phase synthesized conjugates, and improved methods of conjugating pre-synthesized synthetic binding units and nucleic acids. The present invention also relates to the use of conjugates of synthetic binding units and nucleic acids as substrates for various enzymatic reactions, including nucleic acid amplification reactions.

Enzymatic modification of a nucleic acid-synthetic binding unit conjugate

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Wiesbaden, Germany

Stefan Raddatz