Twist Bioscience Corporation

Reseachem Gmbh

Spirochrome Ag

Stefan Pitsch

Bill James Peck

Stefan Berger

Luc Reymond

Patrick A Weiss

Mary Noe

Kai Johnsson

Michelle Frei

Methods, systems, compositions, and devices for the manufacturing of high-quality building blocks, such as polynucleotides, are described herein. Processes described herein provide for efficient washing of residual reagents, solvents, or byproducts from previous synthetic steps to allow for the generation of polynucleotides with low error rates. Processes described herein also provide for reduction in deletion rates during chemical nucleic acid synthesis. Further, methods and devices described herein allow for the rapid construction and assembly of large libraries of highly accurate polynucleotides.

Highly accurate de novo polynucleotide synthesis

The invention relates to a compound characterized by general formula (100), wherein Rand Rare H or F, R, R, Rand Rcan be any substituent, R, R, R, R, Rand Rare a hydrocarbon moiety, one of Rand Ris hydrogen and the other one is hydrogen or a saturated carbon atom connected to any substituent, and its use in staining and live cell fluorescence imaging.

Tunable photoactivatable silicon rhodamine fluorophores

The invention features protected linker compounds which comprise at one terminus a protected amino group and at another other terminus a phosphorous activating group, such as a phosphoramidite group. These protected linker compounds are introduced chemically at the 5'-end of oligonucleotides for the purpose of preparing 5&#x2032;-amino modified oligonucleotides. After deprotection, the thereby introduced amino group then allows further modification (e.g. attachment of dyes) or immobilization (on surfaces or beads) of the oligonucleotide. Specifically, the presented amino protecting group is designed to provide such protected linker compounds in a solid form, which facilitates efficient purification by precipitation or crystallization and aliquoting for distribution and storage.

Protected linker compounds

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Stein am Rhein, Switzerland

Stefan Pitsch