Qiagen Gmbh

Nanogen Recognomics Gmbh

Stefan Pitsch

Sebastian Volker Wendeborn

Albert Eschenmoser

Ioanna Andreou

Luc Reymond

Evelyne Muller

The present invention refers to a double-stranded siRNA molecule comprising a sense Strand and an antisense Strand which is essentially complementary to the sense Strand, each of the sense and the antisense Strands comprising at least 17 nucleotides (nt), the siRNA further comprising at least one overhang at the 5' and/or 3&#x2032; end, wherein the overhang residue or overhang residues are chemically modified and selected independently from each other from the group consisting of: (a) 2&#x2032;-deoxy modified nucleotides; (b) 2&#x2032;-methoxy modified nucleotides; (c) two nucleosides linked by a 3&#x2032; to 5&#x2032; or 2&#x2032; to 5&#x2032; formacetal linkage; (d) nucleotides modified at the 2&#x2032;-position by a &#x2014;O&#x2014;CH&#x2014;O&#x2014;(CH)&#x2014;OH group; and (e) nucleotides comprising in the 3&#x2032;-position a &#x2014;CH&#x2014;O&#x2014;(CH)&#x2014;CHgroup.

Modified short interfering RNA

Processes for the preparation of 3&prime;,4&prime;-cyclic acetals of pentopyranosylnucleosides, in which the pentopyranosyl nucleoside is reacted with an aldehyde, ketone, acetal, or ketal under reduced pressure of less than about 500 mbar.

Method for the production of pentopyranosyl nucleosides

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Zurich, Switzerland

Stefan Pitsch