Sartorius Stedim Biotech Gmbh

Stefan Obermann

Reinhard Baumfalk

Oscar-Werner Reif

Lars Boettcher

Martin Dahlberg

Rainer Sandrock

Isabelle Gay

Matthieu Stettler

Martin Jordan

Dieter Schmidt

Florian Wurm

Maria De Jesus

Thomas Regen

Marc Hogreve

Lars Böttcher

Christian Schmelig

Kenneth P Clapp

Len Goren

A bioreactor container comprising has an at least locally flexible wall and at least one container opening. The wall of the bioreactor container has a fluid-tight inner sheet, and an at least locally fluid-permeable or structured outer sheet. A method for testing the integrity of the bioreactor container also is provided.

Bioreactor container and integrity check method for bioreactor containers

A method for testing the integrity of a bioreactor container includes providing a bioreactor container having a fluid-tight, at least locally flexible wall and having at least one container opening and providing a test apparatus having a bioreactor container compartment and a fluid-permeable and/or structured reception sheet arranged replaceably on the bioreactor container compartment. The method continues by arranging the bioreactor container in the bioreactor container compartment so that the bioreactor container wall contacts the reception sheet in the bioreactor container compartment, connecting the container opening of the bioreactor container to a fluid source filling the bioreactor container with a fluid from the fluid source to generate a predetermined positive pressure Pin the bioreactor container at a first time Tand determining whether the bioreactor container is sufficiently leaktight.

Test method and apparatus for bioreactor containers having reception sheet clamped between bioreactor container and bioreactor container compartment

A method for testing a flexible container includes arranging the flexible container on a winding core that can be rotated about a winding axis W and forms an internal support. The method continues by winding the flexible container around the winding axis W arranging an external support around the wound container and connecting at least one container opening of the container to a fluid source. The method proceeds by filling the container with a fluid from the fluid source to generate a predetermined positive pressure P1 in the flexible container at a first time Tand determining whether the container is sufficiently leaktight.

Method and device for inspecting flexible container

An incubator () has an incubation chamber () and a shaking device () to shake receptacles () placed in the incubation chamber (). A device chamber () adjoins the incubation chamber () and accommodates at least parts of the shaking device (). The shaking device () has a base plate () that seals off the incubation chamber () from the device chamber. A shaking table () is on the inner face () of the base plate () and can be moved in a horizontal plane by a drive arm (). A motor () is arranged on the outer face () of the base plate (). The motor () drives a drive shaft () that is rotatably mounted in the base plate () and that is operatively connected to the drive arm ().

Incubator comprising a shaking device

The invention relates to a device for agitating media (), which are located on a platform (). Said device consists of a housing () with an agitation mechanism, which is used to agitate the platform () and at least one sensor (), which is connected to a control unit () that is equipped with an electronic sensor system (). According to the invention, the sensor(s) () and the electronic sensor system () are located on the platform () and communicate in a wireless manner with the fixed control unit ().

Device for agitating media

A disposable container is provided for mixing media. The container has at least one closable opening for introducing and/or removing media and has sensor mounts at spaced apart locations on the wall of the container. Each sensor mount has a front surface facing into the container and a rear surface sealed against the wall of the container. Sensors are embedded in the respective sensor mounts at positions adjacent the front surfaces. The sensors measure at least one specified parameter of the media at spaced apart locations in the container. The seals between the rear surfaces of the sensor mounts and the wall of the container ensure sterility for the media in the container.

Disposable container having sensor mounts sealed to the container and sensors in the sensor mounts for measuring at least one parameter of media in the container

A method is provided for mixing media in a container. The method includes introducing a first media into the container and then introducing a second media into the container while mixing the media to form a mixture. The method continues by measuring the values of two parameters of the mixture at spaced apart locations in the container. The introduction of the second media into the container may be interrupted based on a comparison of the measured values to a preselected intended value.

Method for mixing media in a container

The invention relates to a device for detecting a sample in a longitudinal sample container (), comprising a sample container holder () for holding the sample container in a housing. Said sample container holder comprises a side viewing window in the region of at least one longitudinal side of the sample container, and a front viewing window () in the region at least one front side of the sample container. Said device also comprises a first illumination arrangement for illuminating the sample container through the front viewing window, a second illumination arrangement for illuminating the sample container through the side viewing window, and an imaging photodetector for detecting a first image of the sample illuminated by means of the first illumination arrangement, and a second image of the sample illuminated by means of the second illumination arrangement. Said detection takes place through the side viewing window. Said device further comprises a digital data processing unit for overlaying the first and second images and for representing the resulting overlay image on a display device connected to the data processing unit.

Method and detection device for the imaging detection of a sample

The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel () until the solid content present in the sample has settled as a compressed cell cake (), and, in order to determine the proportions, the volumes of differently colored sections () of the compressed cell cake () are determined as a measure of the proportions of cells of different viability.

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Adelebsen, Germany

Stefan Obermann