Basf Aktiengesellschaft

Basf Aktiengeselschaft

Stefan Marcinowski

Axel Sanner

Chung-Ji Tschang

Hans-Helmut Goertz

Hardo Siegel

Heinz Hillen

Norbert Greif

Wolfgang Ladner

Christoph Martin

Werner Kuesters

Elard Jacob

Karsten Henco

Gerhard Schenk

Rolf Lungershausen

Peter Moesta

A description is given of a genertic engineering process for the preparation of mesophilic microorganisms which contain a hydantoinase active at elevated temperature, and of DNA sequences which code for this enzyme.

Preparation of mesophilic microorganisms which contain a D-hydantoinase

Enzymes are immobilized by mixing the following components with one another in aqueous solution:

Immobilization of enzymes with a water-soluble glycidyl ether

An insoluble biocatalyst is prepared by forming a mixture consisting essentially of the biocatalyst, one or more water-soluble compounds having two or more acrylamide or meth-acrylamido groups and one or more water soluble amines possessing two or more hydrogen atoms bonded to an amine nitrogen, and forming a gel from the mixture. The water-soluble amine is selected from ammonia, lower diamines, lower polyamines, primary monoamines, polyethyleneimine and polyvinylamine. Beads containing the biocatalyst may be formed by suspending the mixture in a water-immiscible solvent to form droplets prior to gelling. The biocatalyst may be an enzyme, cells or cell fragments.

Preparation of an insoluble biocatalyst

The purification of hTNF by ion exchange chromatography is described.

Purification of hTNF

A macroporous, hydrophilic enzyme carrier comprises a sulfonated polymer, charged with polyethyleneimine, of from 10 to 100% by weight of divinylbenzene, from 0 to 90% by weight of styrene and from 0 to 20% by weight of a copolymerizable monomer, the polyethyleneimine being neither crosslinked nor covalently bonded to the polymer.

Macroporous, hydrophilic enzyme carrier

A biologically active protein which is bonded to a water-insoluble porous copolymer based on N-vinylimidazole and/or substituted N-vinylimidazoles and monomers which can be copolymerized therewith, its preparation and its use for carrying out enzymatic reactions.

Water-insoluble protein material, its preparation and its use

A process for the preparation of D-carbamyl-.alpha.-aminoacids of the formula ##STR1## where R has the meanings given in the description, by enzymatic cleavage of the corresponding hydantoins by means of thermophilic, non-sporulating hydantoin-cleaving micro-organisms or of extracts therefrom.

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Ludwigshafen, Germany

Stefan Marcinowski