Aventis Research & Technologies Gmbh & Co Kg

Stefan M&uuml;llner

Christoph H&uuml;ls

Thomas Neumann

Karl-Christian Gallert

Britta B&ouml;hnisch

A method for disrupting frozen cells and/or tissue in a solid, frozen state in the presence of a solid denaturing substance is provided. The method comprises providing frozen cells and/or tissue in frozen solid form, adding thereto the solid denaturing substance in sold form, and applying mechanical force thereto for disruption of the frozen cells and/or tissue. The frozen cells and/or tissue include muscle cells and/or muscle tissue, wheat cells and/or wheat tissue, and maize cells and/or maize tissue. Further, the solid, denaturing substance in solid form is a crystalline substance and can be selected from urea, thiourea, guanidium chloride, guanidium thiocyanate and ammonium sulfate. Also the solid, denaturing substance in solid form is added to the frozen cells and/or tissue in frozen form in an approximately 1 to 20 fold (w/w) excess.

Disrupting cells and/or tissue in solid state in presence of solid denaturing substance

The invention relates to an insect cell vector for the production of proteins from the DEAD protein family.

Growing community of inventors

Langenfeld, Germany

Stefan Müllner

Growing community of inventors

Langenfeld, Germany

Stefan M&uuml;llner

Stefan Müllner