Plant Genetic Systems N.v.

Bayer Bioscience N.v.

Basf Agricultural Solutions Seed US LLC

Bayer Cropscience Nv

Bayer Cropscience Ag

Bayer Cropscience Gmbh

Aventis Cropscience Gmbh

Aventis Cropscience, N.v.

Stefan K Jansens

Marnix Peferoen

Bart J Lambert

Katrien Van Audenhove

Jeroen Van Rie

Roel Van Aarssen

Hal Moser

Frank Meulewaeter

Arlette Reynaerts

Wendy Aartsen

Ginger Light

Peter Denolf

Rozemarijn Dreesen

Jan Dittgen

Udo Matthias Bickers

Kathleen D'Halluin

Ruediger Hain

Linda Trolinder

Veronique Gossele

Thomas Wilde

Sara Van Houdt

Jonas Vanhaelen

Bernadette Saey

Miriam Hauben

Gary Henniger

Koen Weterings

Van RIE;Jeroen

The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific herbicide tolerance transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.

Elite event EE-GH7 and methods and kits for identifying such event in biological samples

The present invention relates to novel DNA sequences encoding insecticidal Cry1 C proteins derived from, and their use in plants to control insect pests. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising a Cry1 C chimeric gene of the invention and at least one other chimeric gene, such as a chimeric gene encoding an insecticidal Cry1Ab protein, and methods of making or using such plant cells or plants.

Genes encoding insecticidal proteins

Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1&#xd7; of at least one HPPD inhibitor, and methods for making such plants.

Methods and means to modify a plant genome

Four novelstrains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such asspp. and, against Pyralidae such as, and against Yponomeutidae such as, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

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The invention relates to a method for producing plants in which expression of an insecticidal protein is regulated by a wound-induced promoter inducing local expression to the chimeric genes used in this method and the plants obtained thereby, and to the processes for obtaining resistance to insects feeding on plants by localized expression of an insecticidal protein induced on wounding of plants by insect feeding.

Wound-inducible expression in plants

Four novelstrains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to, more particularly against Noctuidae such asspp. and, against Pyralidae such as, and against Yponomeutidae such as, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting

The present invention relates to a DNA sequence encoding a modified Cry1Ab protein that has insecticidal activity. The invention further relates to a method for producing insect resistant plants by introducing into the genome of the plants a foreign DNA comprising such a modified cry1Ab coding sequence. The invention further relates to plants or parts thereof comprising in their genome the modified cry1Ab coding sequence of the present invention.

Plants made insect resistant by transformation with a nucleic acid encoding a modified Cry1Ab protein and methods for making same

Four novel strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and , against Pyralidae such as , and against Yponomeutidae such as , and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

Bacillus thuringiensis strains and their insecticidal proteins

The present invention relates to transformed plants and plant cells comprising DNA molecules encoding proteins with insecticidal activity. The invention relates more particularly to transformed plants and plant cells comprising DNA molecules encoding the protease resistant toxins BTS02618Aa or BTS02618Ab, as well as to methods of rendering plants or plant cells resistant to insects using these DNA molecules.

The present invention relates to transformed microrganisms comprising DNA molecules encoding Bacillus thuringiensis proteins with insecticidal activity. The invention relates more particularly to transformed microrganisms comprising DNA molecules encoding the protease resistant toxins BTS02618Aa or BTS02618Ab.

Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as spodoptera spp. and Agotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains nd that encodes its respective toxin, is resistant to Lipidoptera. Each strain, itself, or its crystal, crystal protein, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combating Lepidoptera.

New modified Cry proteins, particularly modified CryIIIA proteins, having significantly altered toxicity, and DNA sequences encoding these proteins, are designed. Analysis of solvent-accessible amino acid positions in domain II of the CryIIIA protein by alanine-scanning mutagenesis identified individual amino acids involved in corn rootworm toxicity. Random replacement of these amino acids identifies modified proteins with improved toxicity. A combination of all or most thus identified improved amino acids in a single protein yields modified CryIIIA proteins with significantly improved toxicity. Particularly regions protruding from the Cry molecule and located at the apex of the Cry proteins, were identified as involved in toxicity.

Growing community of inventors

Gent, Belgium

Stefan K Jansens