Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Stefan Jakobs

Stefan W Hell

Christian Wurm

Vladimir Belov

Christian Eggeling

Volker Westphal

Mariano Bossi

Kirill Kolmakov

Gael Moneron

Gyuzel Mitronova

Lars Meyer

Vladimir N Belov

Christian Ringemann

Marcel Lauterbach

Claudia Geisler

Vadim P Boyarskiy

Jakob Bierwagen

Martin Andresen

Tanja Brakemann

Christian A Wurm

The present invention relates to novel fluorinated 3,6-diaminoxanthene compounds derived from the basic structural formula (I) and to their uses as photostable fluorescent dyes, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional microscopy, stimulated emission depletion (STED) reversible saturable optically linear fluorescent transitions (RESOLFT) microscopy, and fluorescence correlation spectroscopy. The claimed compounds are also useful as molecular probes in various spectroscopic applications.

Fluorinated rhodamines as photostable fluorescent dyes for labelling and imaging techniques

The present invention relates to novel photoactivable rhodamine or carbopyronine derivatives of the following general formulae G1-G4 (I), G1: A1=O, A2=N, A3=C; G2: A1=S, A2=N, A3=C; G3: A1=O, A2=O, A3=N; G4: A1=S, A2=O, A3=N; comprising UV light absorbing chromophores which after photolysis by irradiation at 254-490 nm (preferably at 375-420 nm) generate fluorescent rhodamine or carbopyronine derivatives and small non-toxic fragments such as Nor NO. The invention also relates to methods for preparing such compounds and to the use of these compounds for optical microscopy and imaging techniques. 

Photoactivable fluorescent dyes for optical microscopy and imaging techniques

The invention relates to novel and improved photostable rhodamine dyes of the general structural formulae I or II and their uses as fluorescent markers, e.g. for immunostainings and spectroscopic and microscopic applications, in particular in conventional and stimulated emission depletion (STED) microscopy and fluorescence correlation spectroscopy. The partially deuterated analogues are useful as molecular mass distribution tags in mass spectroscopic applications, wherein R=an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group; R=H, an unsubstituted or substituted alkyl group, including a cycloalkyl group, or heterocycloalkyl group, or an unsubstituted or substituted aryl group or heteroaryl group, or any combination of such groups; X=CH, C&#x2550;O, C&#x2550;NOR, C&#x2550;NNRNR, CH(OR), O, S, SO, SO, or any other derivatives of these groups, with Ra and Rb independently being H or an organic residue, in particular an unsubstituted or substituted (cyclo)alkyl group or heterocycloalkyl group, an unsubstituted or substituted aryl group or heteroaryl group; Z=a negatively charged group with 1, 2, 3, 4 or 5 charges per anion. 

Hydrophilic and lipophilic rhodamines for labelling and imaging

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Goettingen, Germany

Stefan Jakobs