Icon Genetics Gmbh

Icon Genetics Ag

Stefan Herz

Christian Eibl

Stefan Mühlbauer

Hans-Ulrich Koop

Yuri Gleba

Victor Klimyuk

Sylvestre Marillonnet

Stefan Werner

Carola Engler

Timothy J Golds

Sebastian Klaus

Fong-Chin Huang

Stefan Muehlbauer

A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with anstrain in the presence of a complementing factor, saidstrain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein saidstrain is provided with a first genetic modification rendering saidstrain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.

Biologically safe transient protein expression in plants

The invention provides processes for generating transgenic plants or plant cells transformed on their plastome, comprising introducing into plant plastids a first and a second DNA molecule, wherein the first DNA molecule contains a first region homologous to a region of the plastome for directing plastome integration and a first sequence of interest, and the second DNA molecule contains a second region homologous to a region of the plastome and a second sequence of interest, whereby a sequence segment of the first sequence of interest is homologous to a sequence segment of the second sequence of interest, and selecting transformants having an integration sequence stably integrated in the plastome, whereby the integration sequence contains at least a portion of the first and second sequences of interest as a continuous sequence. The invention further provides plant cells, plants, and seeds of plants produced by such processes.

Plastid transformation using modular vectors

A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.

Processes and vectors for plastid transformation of higher plants

Growing community of inventors

Munich, Germany

Stefan Herz