Basf Se Corporation

Henkel Ag & Company, Kgaa

Stefan Evers

Christian Nitsch

Peter Schmiedel

Karl-Heinz Maurer

Susanne Wieland

Johannes Bongaerts

Christian Degering

Thorsten Eggert

Thomas Weber

Timothy O'Connell

Bernhard Banowski

Hendrik Hellmuth

Nina Mussmann

Wolfgang Von Rybinski

Albrecht Weiss

Cornelius Bessler

Gerhard Gottschalk

Birgit Veith

Marcus Claas

Petra Siegert

Ulrich Schwaneberg

Regina Stehr

Jörg Feesche

Armin Ehrenreich

Heiko Liesegang

Anke Henne

Christina Herzberg

Marion Merkel

Ronny Martinez

Lukas Maksym

Brian Laufs

Felix Jakob

Ayhan Aydemir

Rolf Bayersdörfer

Thomas Gassenmeier

Tobias Kueppers

Victoria Steffen

Renee Charlott Eichstadt

Ramona Knab

Renée Charlott EICHSTÄDT

Timothy O'CONNELL

Cornelius Besseler

Tobias KÜPPERS

Victoria Radnai

Anke Singer

Nina Hoven

Kerstin Sauter

The invention relates to an antiperspirant cosmetic agent which includes at least one specific chitosan and which is free of aluminum-containing compounds, in particular free of halides and/or hydroxy halides of aluminum and/or zirconium. The present invention further relates to the use of a specific chitosan and to a non-therapeutic method for reducing body perspiration. Adding or using the at least one specific chitosan ensures that the sweat gland(s) is/are effectively influenced, thus resulting in a significant reduction in underarm perspiration even in the absence of antiperspirant aluminum-containing compounds.

Chitosan-containing antiperspirant cosmetic agents which are free of halides and/or hydroxy halides of aluminum and/or zirconium

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Expression vectors with promoter and nucleic acid

The present invention relates to an antiperspirant cosmetic agent including at least one specific protein from legumes of the genusand/orand/orand/oror from cruciferous plants of the genusand including no aluminum and/or zirconium halides and/or hydroxyhalides. The present invention further relates to the use of a specific protein and to a non-therapeutic method for reducing body perspiration. Adding or using said at least one specific protein ensures that the sweat gland(s) are effectively influenced, thus resulting in a significant reduction in axillary sweat even in the absence of antiperspirant aluminum salts.

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The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a promoter sequence and a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Expression vectors for an improved protein secretion

In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the specieswhich comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.

Expression method

The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.

Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme

The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.

The present invention relates to 25 hitherto undescribed genes ofand gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

The invention relates to five or four novel genes and the gene products thereof fromand sufficiently similar genes and proteins which are involved in vivo in the formation of polyamino acids. The gene in question is ywsC, ywsC', ywtA, ywtB and ywtD or proteins coded thereby. The gene ywsC, ywsC&#x2032;, ywtA and ywtB can be used to improve biotechnological production methods by microorganisms, wherein they are functionally inactivated; the gene ywtD which codes for a peptide decomposing poly-gamma glutamate can, inversely, contribute to the improvement of biotechnological production methods by increased expression. Said genes can be used positively, preferably to result in a modification or decomposition of poly-gamma glutamate.

Microorganisms providing novel gene products forming or decomposing polyamino acids

Growing community of inventors

Mettmann, Germany

Stefan Evers