Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts

Behring Diagnostics Gmbh

Stefan Dubel

Melvyn Little

Frank Breitling

Iris Klewinghaus

Michael Braunagel

Sergey Kipriyanov

Patrick Fuchs

Thomas Seehaus

Frank Berthold Breitling

Gerd Moldenhauer

Claudia Haas

Khashayarsha Khazaie

Schirrmacher Volker

Stefanie Gotter

Hans-Jurgen Rode

A phagemid has been constructed that expresses an antibody fused to coliphage pIII protein. The phagemid is suitable for selecting specific antibodies from large gene libraries with small quantities of antigen. The antibody-pIII gene can be strongly repressed, so that it allows antibody libraries to be amplified without the danger of deletion mutants predominating. After induction, large quantities of the fusion protein may be expressed.

Phagemid library for antibody screening

Phagemid for antibody screening

Cell surface protein and effector cell bonding reagent

The invention relates to the preparation and use of gene banks of synthetic human antibodies (huAb) or parts of antibodies which contain the antigen-binding domain. Starting from a huAb framework in a suitable vector, the hypervariable regions of the antibody cDNA are formed by almost 'randomly' combined oligonucleotides. Relatively conserved amino acids in the hypervariable regions have here been taken account of in the choice of appropriate nucleotides during the oligonucleotide synthesis and the ratio of the nucleotides used is likewise chosen such that a nonsense codon is to be expected at most in every 89th position. Expression of this synthetic huAb cDNA in microbial expression systems, e.g. in E. coli in the vector pFMT which is described below, thus makes a synthetic huAb library with a comprehensive repertoire for screening using selected antigens available in vitro.

Preparation and use of gene banks of synthetic human antibodies

Recombinant antibodies at the surface of E. coli

Growing community of inventors

Heidelberg, Germany

Stefan Dubel