Ruprecht-karls-universitat Heidelberg

Friedrich-schiller-universitat Jena

Forschungszentrum Mikroskopie (fzm), Luciaoptics Gemeinnützige Ug

Stefan Dithmar

Christoph Cremer

Gerrit Best

Rainer Heintzmann

Roman Amberger

Thomas Ach

Sabrina Rossberger

A fluorescence microscope for obtaining super-resolution images of a sample labeled with at least one fluorescent label by combining localization microscopy and structured illumination microscopy is provided. The fluorescence microscope includes one or more light sources, an illumination system having a structured illumination path for illuminating the sample with structured illumination light and a localization illumination path for illuminating the sample with localization illumination light.

Method and apparatus for combination of localization microscopy and structured illumination microscopy

A method for non-invasive observations of a fundus using an ophthalmoscope is provided. The method includes illuminating a retinal region of an eye by projecting an illumination pattern of illumination light onto the retinal region, at least one of detecting a portion of fluorescent light emitted from the retinal region and detecting a portion of illumination light reflected from the retinal region, thereby capturing a series of images of the retinal region at a plurality of different relative positions of the retinal region with respect to the illumination pattern projected onto the retinal region, wherein between the capturing of at least two images of the series the relative position of the retinal region with respect to the illumination pattern projected onto the retinal region is shifted in a non-controlled manner, and processing the captured images to extract a sub-resolution image of the retinal region.

Structured illumination ophthalmoscope

Growing community of inventors

Dossenheim, Germany

Stefan Dithmar