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Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts

Dade Behring Marburg Gmbh

Stefan D&uuml;bel

Melvyn Little

Iris Klewinghaus

Frank Breitling

Michael Braunagel

Thomas Seehaus

Frank Berthold Breitling

A phagemid has been constructed that expresses an antibody fused to coliphage pIII protein. The phagemid is suitable for selecting specific antibodies from large gene libraries with small quantities of antigen. The antibody-pIII gene can be strongly repressed, so that it allows antibody libraries to be amplified without the danger of deletion mutants predominating. After induction, large quantities of the fusion protein may be expressed.

Phagemid for antibody screening

The invention relates to the preparation and use of gene banks of human antibodies (Ab). Starting from a mixture of human B-lymphocytes, their mRNA is translated into the cDNA using oligo-dT primers. Subsequently, an amplification of the Ab-specific cDNA by means of polymerase chain reaction (PCR) takes place using suitable oligonucleotide primer sequences. Expression of this amplified Ab-specific cDNA in a bacterial expression vector, e.g. the vector pFMT described below, in,thus makes available a human-antibody library with a comprehensive repertoire for screening selected antigens in vitro.

Preparation and use of gene banks of human antibodies (&ldquo;human-antibody libraries&rdquo;)

Growing community of inventors

Dossenheim, Germany

Stefan Dübel

Growing community of inventors

Dossenheim, Germany

Stefan D&uuml;bel

Stefan Dübel