Novozymes A/s

Other

Novozyme A/s

Steen Troels Jorgensen

Allan Svendsen

Jesper Vind

Kim Borch

Shamkant Anant Patkar

Torben Halkier

Claus Crone Fuglsang

Ib Groth Clausen

John C Royer

Peter Bjarke Olsen

Marianne Thellersen

Jens Sigurd Okkels

Titus Kretzschmar

Jens Tonne Andersen

Michael Dolbjerg Rasmussen

Dorte Aaby Petersen

Poul Erik Pedersen

Jens Toenne Andersen

The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of:

Stably maintained multiple copies of at least two ORF in the same orientation

The present invention relates to a modifiedhost cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.

[object Object]

Amutant host cell derived from a parenthost cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

Lipolytic enzymes

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

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Alleroed, Denmark

Steen Troels Jorgensen