Novozymes A/s

Novozymes, Inc.

Steen Troels Joergensen

Randy M Berka

Sara Maria Landvik

Lisbeth Kalum

Ib Groth Clausen

Peter Bjarke Olsen

Alan Berry

Kenneth Jensen

Torsten Bak Regueira

Michael Thomas

Jens Toenne Andersen

Alexandre Bolotine

Alexei Sorokine

Alla Lapidus

Michael Dolbjerg Rasmussen

Bjarke Christensen

Brian Kobmann

Christina Lund Christensen

Anne Breüner

Tina Kristensen

Irina Victorovna Matveeva

Anne BREÜNER

The present invention relates to prokaryotic host cells and methods for improving expression of a mature protease by co-expression with a propeptide of said protease as two separate polypeptides.

Improving expression of a protease by co-expression with propeptide

Provided herein aremutants having improved transformation efficiency, comprising a disruption to an endogenous gene encoding a restriction modification system protein. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.

Bacterial mutants with improved transformation efficiency

The present invention relates to methods for producing a polypeptide comprising using a signal peptide foreign to the polypeptide, nucleic acid constructs comprising a first and a second nucleotide sequence encoding the signal peptide and the polypeptide and expression vectors and host cells comprising said nucleic acid construct. The signal peptide is the LQ2 peptide which is a hybrid of sequence from the signal peptide of alpha amylase fromand of alpha amylase from

Methods for producing secreted polypeptides

The invention relates to a method of indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level from a library of polynucleotides encoding the same polypeptide of interest, or variants or homologues thereof, in a host cell expression library, the method comprising the steps of: a) providing an expression library in a host cell, said library comprising at least two different open reading frames encoding the same polypeptide of interest, or variants or homologues thereof, each open reading frame being translationally coupled to a downstream gene encoding a screenable or selectable reporter; b) culturing the host cell under conditions conducive to the expression of the polypeptide of interest, or variant or homologue thereof; and c) screening and/or selecting for a host cell having an improved expression level of the translationally coupled reporter when compared to one or more other host cells of the library, thus indirectly identifying a polynucleotide comprising an open reading frame having an improved expression level in the host cell; as well as the expression libraries and host cells comprising said libraries.

Selection of well-expressed synthetic genes

The present invention relates to isolated protein complexes having succinyl-CoA:acetoacetate transferase activity, isolated polypeptide subunits thereof, and isolated polynucleotides encoding the subunits. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and protein complexes.

Polypeptides having succinyl-CoA: acetoacetate transferase activity and polynucleotides encoding same

A Bacillus cell for improved production of a polypeptide of interest and a process for producing a polypeptide of interest are disclosed.

Bacillus protein production cell

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Alleroed, Denmark

Steen Troels Joergensen