Novo Nordisk A/s

Abbott Laboratories Corporation

Novo Nordiskals

Steen Troels Jo Slashedrgensen

Bo Slashedrge Diderichsen

Per Linang Jo Slashedrgensen

Alan P Sloma

William R Widner

Michael David Thomas

Lee Fremont Adams

Catherine M Buckley

David John McConnell

Boerge Krag Diderichsen

Audrey Hobson

A prokaryotic cell expressing a gene of interest and comprising at least two copies of said gene on the chromosome.

Procaryotic cell comprising at least two copies of a gene

The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.

Production of bacillus thuringiensis integrants

A method of amplifying in vivo a DNA sequence B present in a genome of a parent cell, comprising (a) integrating in a genome of said cell a DNA construct comprising the structure C-M-A-D, in which both A and C denote a DNA sequence which is homologous with a genomic DNA fragment either flanking or overlapping the DNA sequence B, the sequence C being located in the opposite end the sequence B as compared to A, D denotes a DNA sequence which is homologous with a genomic DNA fragment located distal for C as compared to B, and M denotes a DNA sequence encoding a selection marker, (b) selecting for cells in which the DNA sequence M has been integrated in the genome, which cells comprise, in any orientation, the structure A-B-C-M-A-D and (c) propagating the cells selected in step (b) under increasing selection pressure to obtain a cell which has obtained an increased number of genomically integrated copies of the DNA sequences B and M.

Method of homologous recombination followed by in vivo selection of DNA

DNA integration by transposition

Amplification of genomic DNA by site specific integration of a

Bacillus promoter derived from a variant of a bacillus licheniformis

A bacterial cell which in its genome carries an integrated non-replicative DNA construct comprising (1) a DNA sequence of interest, (2) a DNA sequence which is homologous with a region of the genome of the cell, and (3) an origin of replication, the DNA construct lacking a functional gene coding for a factor required to initiate replication from the origin of replication.

Stable integration of DNA in bacterial genomes

A process for producing an active lipase enzyme in vitro, comprising mixing an inactive or partly active lipase enzyme with a chaperone molecule and subjecting the mixture to denaturation followed by renaturation to produce the active lipase enzyme.

Process for preparing lipases

Growing community of inventors

Aller.o slashed.d, Denmark

Steen Troels Jo Slashedrgensen