Monsanto Technology LLC

Pharmacia Corporation

Monsanto Company

Mendel Biotechnology, Inc.

Stanton B Dotson

Sam R Eathington

Kevin Lee Deppermann

Michael W Petersen

David V Butruille

John Tamulonis

Bruce J Schnicker

Heather M Forbes

David K Kovalic

Jingdong Liu

Yongwei Cao

Wei Wu

James D McIninch

Linda L Lutfiyya

Andrey A Boukharov

Jingrui Wu

Jeffrey M Koshi

Nengbing Tao

Thomas Gene Ruff

Fenggao Dong

Changlin Fu

Zhidong Xie

Targolli L Jayaprakash

Jindong Sun

Carolyn J Thai

Kottaram Krishnadas Narayanan

Frederic Achard

Allen N Ondes

David W Finley

Rajeev Aurora

William M Fischer

Santanu Dasgupta

Mary Fernandes

Steve Sichuan He

John M Jensen

Zoe P McKiness

Kimberly Zobrist

Oliver J Ratcliffe

Ganesh M Kishore

Jacqueline E Heard

Gregory R Heck

Neal I Gutterson

Cai-Zhong Jiang

Timothy W Conner

Roderick W Kumimoto

Thomas R Adams

Scott E Andersen

Jeffrey M Libby

Frederick D Hempel

Donald E Nelson

Michael D Edgerton

James S Keddie

Bradley K Sherman

Thomas J La Rosa

Garrett J Lee

Ronald B Frazier

Zoe McCuddin

Kimberly Faye Zobrist-Duff

Santana Dasgupta

Carolyn J Sympson

Cynthia L Woods

Hamideh Zakeri

Xianzhi Zhou

Heather Forbes

Santana 101 Spoorthy Apartment Dasgupta

Kottaram K Narayanan

T L Jayaprakash

Steven S He

A method is provided for use in analyzing seeds, in a population of seeds, for one or more desired characteristic. The method includes removing tissue from individual seeds in a population of seeds using an automated seed sampler, while preserving germination viability of the seeds, and analyzing the tissue for the presence or absence of one or more desired characteristic. The method then includes, based on the analysis of the tissue, quantifying the one or more desired characteristic for each of the individual seeds that possess the one or more desired characteristic.

Methods of seed breeding using high throughput nondestructive seed sampling

A method is provided for use in a plant breeding program to generate a population having one or more desired traits. The method includes removing a tissue sample from each of one or more seeds using an automated seed sampler while preserving germination viability of the sampled seeds; analyzing the tissue samples for the presence or absence of a genetic sequence associated with at least one desired trait; based on the analysis of the tissue samples, cultivating plants from the sampled seeds that either possess or lack the genetic sequence associated with the at least one desired trait; creating a parental cross using at least one of the cultivated plants; and selecting seeds from the parental cross and cultivating offspring of the parental cross using the selected seeds.

A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

Automated systems for removing tissue samples from seeds, and related methods

The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.

Methods for sequence-directed molecular breeding

Novel methods are provided to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for introgressive hybridization, for example, generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, and analyzing nucleic acids extracted from the tissue samples for at least one genetic marker. The method then further includes selecting the sampled seeds that possess the at least one genetic marker, cultivating fertile plants from the selected seeds, and crossing the fertile plants with other plants.

The present invention discloses rice genomic promoter sequences. The promoters are particularly suited for use in rice and other cereal crops. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the rice genomic promoter sequences, and methods for preparing and using the same.

Genomic plant sequences and uses thereof

This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

Transgenic plants with enhanced agronomic traits

The present invention provides a method and DNA molecules that when expressed in a plant produces transgenic plants with improved abiotic stress tolerance. The invention includes plant expression vectors comprising the DNA molecules, and plants containing such DNA molecules.

Stress tolerant plants and methods thereof

The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method of evaluating seeds for a desired trait, for example, for facilitating germplasm improvement activities, etc., generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, analyzing the tissue samples for one or more characteristics indicative of a desired trait, and selecting the sampled seeds that include the desired trait.

Disclosed herein are transgenic plants having recombinant DNA which expresses a G1073 transcription factor which provides enhanced resistance and/or tolerance to water deficit. More specifically the DNA constructs comprise a polynucleotide which encodes at least a functional part of a G1073 transcription factor or a homologous transcription factor.

Transgenic plants

The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.

Growing community of inventors

Chesterfield, MO, United States of America

Stanton B Dotson