Exact Laboratories, Inc.

Cytyc Corporation

Fluidigm Corporation

Exact Sciences Corporation

Helicos Biosciences Corporation

Other

The Machlett Laboratories, Incorporated

Itran Corporation

Sequenom, Inc.

Stanley N Lapidus

Anthony P Shuber

Dean Kamen

Doron Lipson

J William Efcavitch

Timothy D Harris

Lewis T Polk, Jr

Philip Richard Buzby

Kevin M Ulmer

Gail E Radcliffe

Fredric L Farber

Phillip Richard Buzby

Walker M Slaon, Iii

George Q Daley

John F Thompson

Anne A Hurley

J Morgan Barlas

Seymour Abraham Friedel

Arlen M O'Lari

Richard R Villeneuve

Joseph J Dziezanowski

Michael P Greenberg

Patrice M Milos

Stanley Letovsky

The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.

Single molecule sequencing of captured nucleic acids

The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.

Short cycle methods for sequencing polynucleotides

The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or fill completion.

Methods are provided for selective nucleic acid sequence detection in single base primer extension reactions of high sensitivity. These methods are useful for detecting small amounts of mutant nucleic acid in a heterogeneous biological sample. These methods are particularly useful for identifying individuals with gene mutations indicative of early colorectal cancer.

Primer extension methods for detecting nucleic acids

The invention provides an apparatus for obtaining a stool specimen. The apparatus comprises a housing, a collection bag, a slider to close the collection bag and a draw string to move the slider. A user places the apparatus on a toilet, deposits a first stool into the collection bag, then pulls the draw string to move the slider to close the collection bag thereby allowing the user to urinate or further defecate.

Stool specimen collector

The present invention provides methods for the preparation of stool samples to increase the yield of relevant DNA, and further provides methods for isolating and analyzing target DNA for characteristics indicative of colorectal cancer. Methods for screening patients for the presence of cancerous or pre-cancerous colorectal lesions are also provided.

Methods for stool sample preparation

An apparatus for obtaining a stool specimen. The apparatus comprises a housing, a collection bag, a slider to close the collection bag and a draw string to move the slider. A user places the apparatus on a toilet, deposits a first stool into the collection bag, then pulls the draw string to move the slider to close the collection bag thereby allowing the user to urinate or further defecate.

The present invention provides methods for preparing a stool sample in order to screen for the presence of indicators of a disease, for example a subpopulation of cancerous or precancerous cells. The methods take advantage of the recognition that cellular debris from cancerous and precancerous cells is deposited onto only a longitudinal stripe of stool as the stool is forming in the colon. Accordingly, methods of the invention comprise obtaining a representative sample, such as a circumferential or cross-sectional sample of stool in order to ensure that any disease indicator, such as cellular debris that is shed by colonic cells, is obtained in the sample.

Methods for disease diagnosis from stool samples

Methods are provided for identifying nucleic acids. Methods of the invention are useful for identifying and analyzing nucleic acids, especially variants of single nucleotide polymorphisms, that are indicative of disease or the predisposition for disease.

Methods for the detection of nucleic acids

Growing community of inventors

Bedford, NH, United States of America

Stanley N Lapidus