Monsanto Technology LLC

Board of Regents of the University of Nebraska

Stanislaw Flasinski

Catherine Alice Chay

James Arthur Baum

Arlene R Howe

David Joseph Bowen

Sara Ann Salvador

Xiaoran Fu

Wei Wu

Thomas Cerruti

James Kevin Roberts

Jay Paul Morgenstern

Leigh H English

Uma R Kesanapalli

Victor M Guzov

Jaishree M Chittoor

Artem G Evdokimov

Andrew M Wollacott

Mohammed Oufattole

Jack Q Wilkinson

Todd A Ciche

Kunsheng Wu

Wen C Burns

Jason S Milligan

Paul C C Feng

Nengbing Tao

Zhaolong Li

Gregory R Heck

Marianne Malven

Charles R Dietrich

Michael W Petersen

Qi Wang

Timothy W Conner

Maolong Lu

Liang Guo

Thomas M Malvar

Xudong Ye

David K Kovalic

Farhad Moshiri

Elysia K Krieger

Linda L Lutfiyya

Andrey A Boukharov

Ty T Vaughn

Yongwei Cao

Shihshieh Huang

Thomas Clark

Murtaza F Alibhai

Jun Zhang

Deryck Jeremy Williams

Michael John Crawford

John D Bradley

Junhua Zhang

Crystal L Dart

Jinling Hung Wang

Yong Yin

Karen L Fincher

Rachael N Slightom

Krishnakumar Sridharan

Megan N Schroder

Xiaohong Shi

Shiaw-Pyng Yang

Barrett C Foat

Mingqi Deng

Randall W Shultz

Xiaoping Wei

Cheryl L Cloninger

Sarah L Brown

Heidi M Windler

Uma Rao Sukuru

Aihong Pan

Jason W Stelzer

Jinsong You

Timothy J Rydel

Eric J Sturman

Peter T Hajdukiewicz

Bo-Xing Qiu

Ervin D Nagy

Heather M Anderson

Moritz Von Rechenberg

Meiying Zheng

Halong Vu

Ping Li

Michelle Lacy

James Morrell

David J Chi

William P Clinton

Robert M McCarroll

Deborah G Rucker

Youlin Qi

James K Roberts

Steven E Screen

William Carlton Stallings

Shirley Xiaoli Guo

Brad Kane Gabor

David J Bowen

Cara L Griffith

John Kao

John W Pitkin

Richard Eric Cerny

Donald P Weeks

Jeffrey R Nageotte

PLANT REGULATORY ELEMENTS AND USES THEREOF

The invention provides a transgenic corn event MON95275, plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, commodity products comprising detectable amounts of corn event MON95275 DNA. The invention also provides polynucleotides specific for corn event MON95275 and methods for using and detecting corn event MON95275 DNA as well as plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising corn event MON95275. The invention also provides methods related to making and using corn event MON95275.

Transgenic corn event MON95275

A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

Insect inhibitory proteins

Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

Lepidopteran-active Cry1Da1 amino acid sequence variant proteins

The present invention discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

Proteins toxic to Hemipteran insect species

Recombinant DNA molecules and constructs including sequences derived fromare provided, which are useful for modulating gene expression in plants. Transgenic plants, plant cells, plant parts, and seeds are also provided, comprising a recombinant DNA molecule operably linked to a heterologous transcribable DNA molecule, as well as methods of their use.

Plant regulatory elements and uses thereof

This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

Compositions and methods for genome editing in planta

Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests

Disclosed herein are nucleotide sequences encoding an insecticidal protein exhibiting Lepidopteran inhibitory activity, as well as novel insecticidal proteins referred to herein as a BCW 001, BCW 002, BCW 003, and BCW toxic protein-containing chimeras and BCW toxin insecticide, transgenic plants expressing the chimeras or the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

Pesticidal toxin proteins active againstinsects

Transgenic corn event MON95275 and methods for detection and uses thereof

The invention provides DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. Transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules. Also provided are methods of the use of the recombinant DNA molecules and constructs and the transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules and constructs.

Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The present invention provides novel DNA molecules and constructs, including their nucleotide sequences, useful for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising the DNA molecules operably linked to heterologous transcribable polynucleotides, along with methods of their use.

Growing community of inventors

Chesterfield, MO, United States of America

Stanislaw Flasinski