The Foundation for the Promotion of Industrial Science

Neuroindx, Inc.

Centre National De La Recherche Scientifique

Centre National De La Recherche Scientifique-cnrs

Stanislav L Karsten

Hiroyuki Fujita

Dominique Collard

Lili C Kudo

Momoko Kumemura

The invention relates to a system () adapted to measure multiple biophysical characteristics of cells, the system () comprising: a microfluidic chip () provided with a microfluidic channel () which allows cells to flow through, the microfluidic channel () having an inlet (), an outlet (), and a lateral opening () situated between the inlet () and the outlet (); and a capacitive sensor () integrated in the microfluidic chip, adapted to obtain biophysical characteristics of a single cell in the microfluidic channel () by directly manipulating the single cell by sensor elements () through the lateral opening () of the microfluidic channel (), the sensor () comprising a stationary part and an electrostatically driven movable part which is movable relative to the stationary part, the stationary part being fixed to the microfluidic chip (), the movable part being arranged in the lateral opening () of the microfluidic channel (), wherein a portion of the sensor elements () provides an interface between fluid and air in the system.

Measuring system and manufacturing process of such a measuring system

An object is to make a bridge of DNA expanding between a pair of electrodes, and to characterize the bridge of DNA, to thereby detect DNA easily and surely without employing any marker or labeling substances, such as fluorescent reagents. A method of detecting DNA using a detection device with at least a couple of electrodes, the method comprising immobilizing a primer on the electrodes; making a bridge of the DNA expanded between the electrodes, by immersing the electrodes in a solution including circular templates of single stranded DNA, annealing the circular templates, and generating single stranded DNA product utilizing RCA (Rolling Circle Amplification), with impressing a designated voltage between the electrodes; and characterizing the bridge of DNA which includes multiple single stranded DNA molecules between the electrodes.

DNA detection method

Microscope straddle stand

Growing community of inventors

Signal Hill, CA, United States of America

Stanislav L Karsten