Ventana Medical Systems, Inc.

Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-La Roche Ag

Stacey Stanislaw

Larry E Morrison

Jerome W Kosmeder, Ii

William Kenneth Day

Michael Otter

Brian Daniel Kelly

Thomas M Grogan

Nelson Alexander

Mark Lefever

Aoune Barhoumi

Anne Pedata

Lisa L Gallegos

Jigar Patel

Benjamin C Stevens

Jan Berka

Esteban Roberts

Sedide Ozturk

Nathan Crum

Alisa Tubbs

Taylor J Shingler

Seoyoung Kim

Rui Chen

Timothy James Keller

Daniel Burgess

Hiro Nitta

Florian Rubelt

Toumy Guettouche

Maeve E O'Huallachain

Toummy Guettouche

Samantha Rajkovich

Melinda Day

Alastair Laing

Eric Walk

Katherine Leith

Vinnie Petre

Elizabeth Pigman

Heidi J Rosenbaum

Leigh A Henricksen

Jerome W Kosmeder

Heidi Rosenbaum

William Day

Nelson R Alexander

Maeve E O'HUALLACHAIN

Marvin Arthur Petre

The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially 'personalized' anti-tumor vaccines or immune cell based therapies.

Representative diagnostics

Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

Signaling conjugates and methods of use

The present disclosure provides a method of separating cellular particles from a tissue sample and then sorting the cellular particles into two or more cellular particle populations.

Size-based separation of dissociated fixed tissues

In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.

Deep sequencing profiling of tumors

The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

System for identification of antigens recognized by T cell receptors expressed on tumor infiltrating lymphocytes

A tissue sample that has been removed from a subject can be properly fixed for evaluation using the disclosed transporter assembly for carrying a tissue sample and method for fixing an unfixed tissue sample. In one embodiment, the disclosed assembly includes a transport container, a fixative in the transport container, and a cooling device that reduces and/or maintains the temperature of the fixative to perform a pre-soaking process at a temperature of less than about 7&#xb0; C. The pre-soaking process can, for example, be performed during sample transport or during extended periods of storage, such as over a weekend.

Transporter systems, assemblies and associated methods for transporting tissue samples

Growing community of inventors

Tucson, AZ, United States of America

Stacey Stanislaw