Creighton University

Streck, Inc.

Stacey Morrow

Nancy D Hanson

Matthew R Kreifels

Joel Lechner

Stephanie Cossette

Maria Torres-Gonzalez

Randy Fowler

Laura R Porter

Cathy Scheer

Christopher Connelly

Hayden Smith

Chris Connelly

Maria Torres

Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple β-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of β-lactamase genes selected from the group consisting of one or more of the following: MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51-like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

Assays and methods for determining microbial resistance

Systems and methods for detection and classification of IMP carbapenemases are described. In an aspect, sets of PCR primers associated with specific probes can be used to identify specific families of IMP β-lactamases and utilize multiple primer sets to identify all IMP variants via real-time PCR assays. The sets of PCR primers can also be coupled with a fluorescent dye to detect a broad range of the IMP variants.

Detection and classification of IMP carbapenemases using real-time polymerase chain reaction assay

Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple &#x3b2;-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of &#x3b2;-lactamase genes selected from the group consisting of one or more of the following: MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51-like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

Growing community of inventors

Omaha, NE, United States of America

Stacey Morrow