Harvard College

Joule Unlimited Technologies, Inc.

Joule Unlimited, Inc.

Sriram Kosuri

David Arthur Berry

Noubar Boghos Afeyan

Dan Eric Robertson

Frank Anthony Skraly

Christian Perry Ridley

Brian D Green

George M Church

Nikos Basil Reppas

Martha Sholl

Feng Zhang

Le Cong

Richard C Terry

Di Zhang

Eric James Devroe

Brian Green

Eric Devroe

Provided herein are compositions, kits and methods useful in the construction of designer transcription activator-like effector (dTALE) polypeptides.

Transcription activator-like effectors

Methods and compositions for synthesizing nucleic acid sequences in an emulsion are provided.

Assembly of nucleic acid sequences in emulsions

The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.

Engineered CO2 fixing microorganisms producing carbon-based products of interest

The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, alkanes, isoprenoids, pharmaceutical products or intermediates thereof.

The present disclosure identifies pathways and mechanisms to confer improved industrial fitness on engineered organisms. It also discloses engineered organisms having improved industrial fitness. Synthetic biologic engineering modules are disclosed that provide for light capture, carbon dioxide fixation, NADH production, NADPH production, thermotolerance, pH tolerance, flue gas tolerance, salt tolerance, nutrient independence and near infrared absorbance. The disclosed engineered organisms can include one or more of these modules. Also provided are methods of using the engineered organism to produce carbon-based products of interest, biomass or pharmaceutical agents.

Hyperphotosynthetic organisms

Growing community of inventors

Cambridge, MA, United States of America

Sriram Kosuri