Combangio, Inc.

Xcella Biosciences, Inc.

Leland Stanford Junior University

Spencer Caleb Alford

Jennifer R Cochran

Bob Chen

Audrey Smith

Thomas Michael Baer

Ivan Krastev Dimov

Arvind Kannan

Sungwon Lim

The present application provides methods and processes for making and using a lyophilized mesenchymal stem cell secretome, as well as methods for treating ocular conditions and/disorders with the reconstituted lyophilized mesenchymal stem cell secretome described herein.

Lyophilized mesenchymal stem cell derived secretome and uses thereof

The present application provides methods and processes for making and using a fibronectin composition, as well as methods for treating ocular conditions and/or disorders with the cellular fibronectin composition described herein.

Processes for making a cellular fibronectin composition

High-throughput methods for screening large populations of variant proteins are provided. The methods utilize large-scale arrays of microcapillaries, where each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be isolated, and cells expressing the variant proteins of interest can be characterized. Also provided are systems for performing the disclosed screening methods.

Methods and systems for screening using microcapillary arrays

Methods for treating an ocular condition with cellular fibronectin compositions

Cellular fibronectin compositions

The present application provides methods and processes for making and using a mesenchymal stem cell secretome, as well as methods for treating ocular conditions and/disorders with the mesenchymal stem cell secretome described herein.

Processes for making and using a mesenchymal stem cell derived secretome

Microcavity arrays and methods for quantitative biochemical and biophysical analysis of populations of biological variants. Examples include high-throughput analysis of cells and protein products use a range of fluorescent assays, including binding-affinity measurement and time-resolved enzyme assays. Laser-based extraction of microcavity contents.

Growing community of inventors

Mountain View, CA, United States of America

Spencer Caleb Alford