Cristalia Produtos Quimicos Farmaceuticos Ltda.

Spartaco Astolfi Filho

Ogari De Castro Pacheco

Roberto Carlos Debom Moreira

Lucidio Cristovao Fardelone

Josef Ernst Thiemann

Marcos Castanheira Alegria

Marina Baiochi Riboldi Delalana

Lucidio Cristóvão Fardelone

The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus. Particularly, the present invention refers to an animal product-free culture medium for, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producingliquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.

Animal product-free culture medium for bacteria of the genusand a process for producing supernatant comprising one or more collagenolytic and gelatinolytic proteases

The present invention refers to a culture medium and a process for producingcollagenolytic and gelatinolytic proteases. The present invention includes an animal product-free culture medium forcomprising non-animal derived peptones, preferably vegetable derived peptones, yeast extract and the amino acids cysteine and arginine. The present invention also includes a process for producing a supernatant ofliquid culture comprising one or more collagenolytic and gelatinolytic proteases, and pharmaceutical composition comprising as active ingredient a supernatant of thecultures described herein.

Animal product-free culture medium and a process for producing a supernatant of clostridium comprising one or more collagenolytic and gelatinolytic proteases

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Itapira, Brazil

Spartaco Astolfi Filho