Pearl Kogyo Co., Ltd.

Bbk Bio Corporation

The Hollenniun Laboratories

Meiryo Technica Corporation, Ltd.

Sousuke Miyoshi

Hiroyuki Arakawa

Susumu Satoh

Shintaro Nishimura

Susumu Sato

Masafumi Koide

Masao Fukagawa

Jyunko Zenkou

Akiko Ohkubo

Yoshitaka Sakamoto

Juichiro Nakashima

Noriyuki Morikawa

Akiko Suzuki

Yasuhiro Ogawa

Hiroshi Marusawa

A method of highly efficiently transferring various selected molecules into various cells and a method of fusing cells. Cells and/or selected molecules such as polynucleotide are treated with cold gas plasma to thereby transfer the selected molecules located around cells into the cells, or cells are fused by treating the cells with cold gas plasma. Moreover, an apparatus for transferring selected molecules or fusing cells having a cold gas plasma generation unit for transferring selected molecules into cells is provided.

Method of transferring a selected molecule into a cell

A molecule introducing apparatus is provided with a power supply circuitfor supplying power, a step-up transformerwhich is supplied with power from the power supply circuitand which outputs a high voltage, a switching transistorthat blocks off and allows the supply of power from the power supply circuitto the step-up transformerand which generates an instantaneous high voltage, and a pair of electrode probesfor applying the instantaneous high voltage generated at the step-up transformerto a predetermined region in a body. The molecule introducing apparatus oscillates DNA arranged outside a cell by the instantaneous high voltage so as to introduce the DNA into the cell.

Molecule transferring device, auxiliary for molecule transferring device, and molecule transferring method

Growing community of inventors

Osaka, Japan

Sousuke Miyoshi