The Brigham and Women's Hospital, Inc.

The Broad Institute, Inc.

Soumyashree Ashok Gangopadhyay

Amit Choudhary

Basudeb Maji

Kurt Cox

Miseon Lee

Debasish Manna

Donghyun Lim

Mengchao Shi

The disclosure includes non-naturally occurring or engineered DNA- or RNA-guided nuclease systems, comprising CRISPR enzymes associated with at least one destabilization domain (DD) and photocaged stabilization ligands with at least one photocage molecule, along with compositions, systems and complexes involving such systems, nucleic acid molecules and vectors encoding the same, delivery systems involving the same, uses thereof.

Methods and compositions for optochemical control of CRISPR-CAS9

Compositions and methods for inhibiting the activity of RNA-guided endonucleases, and methods for identifying such compositions are included. Methods of treatment using the inhibitory compounds, formulations and methods are also detailed, as well as control of nucleic acid editing systems and methods using said compounds.

Inhibitors of RNA-guided nuclease target binding and uses thereof

The present inventions generally relate to site-specific delivery of nucleic acid modifiers and includes novel DNA-binding proteins and effectors that can be rapidly programmed to make site-specific DNA modifications. The present inventions also provide a synthetic all-in-one genome editor (SAGE) systems comprising designer DNA sequence readers and a set of small molecules that induce double-strand breaks, enhance cellular permeability, inhibit NHEJ and activate HDR, as well as methods of using and delivering such systems.

Nucleic acid modifiers

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Boston, MA, United States of America

Soumyashree Ashok Gangopadhyay