Rutgers, the State University of New Jersey

Specific Diagnostics, LLC

Soumitesh Chakravorty

David Alland

Sung Hyun Lim

Paul A Rhodes

The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes infor identifying the presence ofDNA and/or resistance to anti-tuberculosis drugs.

Polymerase chain reaction primers and probes for

Devices, systems, and methods for strain-specific identification and assessment of susceptibility of microorganisms based on the response of sensors in a colorimetric sensor array to metabolic products of the microorganism. An exemplary method includes culturing a sample containing microorganisms in a medium and in gaseous communication with a colorimetric sensor array. Sensors in the colorimetric sensor array are exposed to volatile organic compounds produced by the microorganism. The method then includes assessing a resistance of the microorganism to at least one substance. The resistance is assessed based on a response of the sensors in the colorimetric sensor array to the volatile organic compounds produced by the microorganism.

Detection of drug resistance of microorganisms

This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistantin a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistantin a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wild-type RRDR indicates the presence of rifampicin resistantin the sample.

Detection of drug resistant mycobacterium tuberculosis

The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes infor identifying the presence of M.tb DNA and/or resistance to anti-tuberculosis drugs.

Detection of drug resistant

This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistantin a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistantin a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wildtype RRDR indicates the presence of rifampicin resistantin the sample.

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Somerset, NJ, United States of America

Soumitesh Chakravorty