Fuso Pharmaceutical Industries, Ltd.

Souji Eda

Akio Matsuhisa

Masahiro Asakura

Shinji Yamasaki

Hirotsugu Uehara

Kanako Abe

Yuichi Oku

Seiji Yamamoto

Yoshihiko Uesaka

Norihiko Sugimoto

Kazumasa Kamei

Yusuke Shibahara

Very simple, highly sensitive detection or quantification of target nucleic acids of interest has been achieved by: hybridizing mask oligonucleotides to regions in a single-stranded region of a nucleic acid to be assayed between which a region to be hybridized by an oligonucleotide probe is positioned, thereby opening the probe-hybridizing region and keeping the single-stranded region of the target nucleic acid stable, and then subjecting this nucleic acid having the single-stranded region to nucleic acid chromatography.

Nucleic acid detection or quantification method using mask oligonucleotide, and device for same

It is intended to provide a method of detecting a nucleic acid whereby a target nucleic acid can be accurately and quickly detected at an elevated detection sensitivity compared with the existing methods; and a gene detection kit with the use of this method. A sample containing cells is fixed to a support and nucleic acids are amplified on the support as such. Then, a nucleic acid thus amplified is detected. Since the nucleic acids are not detected from the sample in this method, a lowering in the detection sensitivity due to the nucleic acid loss in the step of extracting the nucleic acids can be prevented. Since the amplified nucleic acid is detected, furthermore, detection can be made even though the nucleic acid is contained only in a trace amount in the sample.

Method of detecting nucleic acid and utilization thereof

Growing community of inventors

Osaka, Japan

Souji Eda