Kaneka Corporation

Sotaro Sano

Shigehiko Miyamoto

Jun Tomono

Takaaki Jikihara

Koji Takahashi

Hiroyuki Hata

Hajime Hiratsuka

Akiko Kawai

A test device is provided that can comprise: a housing accommodating a chromatography support, wherein the housing comprises: a supporting part that supports a container accommodating a liquid used for chromatography. A method is provided for performing chromatography using the test device.

Chromatographic test device

A test device includes a housing and a lid. The housing encloses an internal space, has a hole supporting a container accommodating liquid, and includes a perforation/incision part. The lid covers a hole-formed part of the housing. The housing and/or the lid includes a guide that guides the housing and the lid, so that the lid can migrate from the first position to the second position while covering the hole-formed part of the housing. In the first position, the lid covers the hole-formed part of the housing and the container and the container is not incised. During the migration of the lid from the first position to the second position, the container is pushed toward the perforation/incision part by the lid, and the container is incised to leak liquid into the internal space.

Test device

Provided is a method for detecting small RNAs in a simple and highly accurate manner. Provided is a nucleic acid detection method including the following steps (a) to (c): (a) carrying out a reverse transcription reaction using a target RNA as a template and a reverse transcription primer having on its 5'-end side a sequence non-complementary to the target RNA to produce a reverse transcription product longer than the target RNA; (b) carrying out a nucleic acid amplification reaction using the reverse transcription product as a template and two primers to produce an amplified double-stranded DNA fragment having a single-stranded region at least at one end; and (c) hybridizing the single-stranded region of the amplified double-stranded DNA fragment to an oligonucleotide probe immobilized on a solid phase.

Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end

An object of the present invention is to provide methods for amplifying and detecting a nucleic acid that allow efficient hybridization, and devices and kits for use in the methods. The present invention includes amplifying a target nucleic acid into a double-stranded nucleic acid having a single-stranded region at each end, and detecting this nucleic acid. The present invention also provides detection devices and kits that make use of these methods.

Method for amplifying nucleic acid and method for detecting amplified nucleic acid

An object of the present invention is to provide a technique for preparing RNA ready for an enzymatic reaction more easily than conventional techniques. The present invention provides a reagent for RNA extraction from a biological sample which contains an alkali metal salt and a surfactant.

RNA preparation method

The present invention aims to solve problems in the analysis of amplified nucleic acids, i.e., cost, workability, and contamination and pollution of samples. In the present invention, a sample containing a nucleic acid and a reagent for detecting the nucleic acid are mixed in a closed system. In this regard, the nucleic acid is amplified in the same closed system, and then mixed with the detecting reagent without opening the system. In order to carry out this method, for example, a device that includes the detecting reagent shielded by a coating material or the like is used.

Nucleic acid detection method, and device and kit for use in same

The present invention provides a method for detecting a nucleic acid, by which a multi-stranded nucleic acid amplified by a nucleic acid amplification method is detected easily and with a high degree of accuracy without the need for specialized equipment, and also provides a nucleic acid detection device. Provided are a method for detecting a nucleic acid under visible light via a color reaction produced by contact between a chromogenic leuco dye and the multi-stranded nucleic acid, as well as a nucleic acid detection device using this method.

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Takasago, Japan

Sotaro Sano