Roche Sequencing Solutions, Inc.

Signature Diagnostics Gmbh

Sophie Beckert

Bernd Hinzmann

Bronwen Miller

Alexander Lovejoy

Daniel Klass

Melissa Loyzer

Victoria Constandse

Present disclosure provides a method including isolating DNA from a source, thereby providing a composition including the isolated DNA. The isolated DNA has at least first and second target regions, where the length of the second target region is greater than the length of the first target region. The method further includes quantifying a total mass of the isolated DNA, quantifying a first quantification cycle (C) of the first target region and a second Cof the second target region, and calculating a Q-ratio for the isolated DNA by dividing the second Cby the first C. The method further includes determining a value for a quality-mass constant (k), estimating a required input mass by dividing kby the Q-ratio, and preparing the isolated DNA for sequencing if the total mass of the isolated DNA in the composition is equal or greater than the required input mass.

System and method for isolation and qualification of nucleic acids

The present invention provides method essentially comprising the steps of (i) providing a DNA sample, (ii) providing at least a first pair of amplification primers which is capable of generating a first amplicon from a LINE sequence that has a size of less than 80 bp, (iii) providing at least a second pair of amplification primers which is capable of generating a second amplicon from a LINE sequence that has a size of more than 160 bp, (iv) performing a qPCR and determining cq values for each of the generated amplicons, and (v) determining the relative concentrations of said amplicons.

Quality testing of DNA samples

Growing community of inventors

Neuzelle, Germany

Sophie Beckert