Regeneron Pharmaceuticals, Inc.

Regeneran Pharmaceuticals, Inc.

Sook Yen E

Haibo Qiu

Ning Li

David Bramhall

The present disclosure provides methods and compositions for the determining the abundance and/or concentration of protein biomarkers in a biological sample.

Use of LC-MS/MS to quantitate protein biomarkers

Compositions and methods for identifying free thiols in protein are provided. An exemplary method labeling peptides with a tag to identify free thiols and a tag to identify native disulfide bonds and analyzing the tags using targeted MS. In one embodiment, the method provides complete coverage of all 32 cysteine residues in an IgG molecule. In other embodiments the method covers the 16 cysteine residues on the heavy and light chains in an IgG molecule. In another embodiment, the method covers the 5 cysteine residues on each light chain of an IgG molecule. In another embodiment, the method covers the 11 cysteine residues on each heavy chain of an IgG molecule.

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Croton-on-Hudson, NY, United States of America

Sook Yen E