Hypharm Gmbh

Hyglos Invest Gmbh

Biomerieux Sa

BiomÉrieux Deutschland Gmbh

Ludwig-Maximilians-Universitat München

Sonja Molinaro

Gerhard Winter

Holger Grallert

Bernd Buchberger

Wolfgang Mutter

Anna Scherzinger

Julia Lorenz

Simon Eisele

Julia Kaps

The present invention relates to assays, kits and oligonucleotides for the detection offor a fast, sensitive and reliable detection ofin a species- and serotype-specific manner. In particular, the present invention provides an assay for the serotype-specific detection of, a kit for the serotype-specific detection of, as well as oligonucleotides useful in such assay or kit. The present invention further relates to the use ofserotype specific antibodies for serotype specific treatment ofinfection in a patient detected for said specificserotype with such an assay or kit.

Chimeric polypeptides and their use in bacterial decolonization

The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.

Method for recombinant production of horseshoe crab Factor C protein in protozoa

The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonisation and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.

Methods of generating and screening for lytic chimeric polypeptides

Method for recombinant production of horseshoe crab factor C protein in protozoa

The present invention relates to novel methods of generating and screening for chimeric polypeptides, which can be used in the treatment and prophylaxis of pathogenic bacterial contamination, colonization and infection. The novel methods are based on random recombination of protein domains, and the chimeric polypeptides obtainable by the methods according to the invention are characterized in that they comprise at least one enzymatic active domain (EAD) and at least one cell binding domain (CBD). The present invention also relates to a library of chimeric polypeptides obtainable by the methods of the present invention.

The present invention relates to chimeric polypeptides comprising a first portion, which comprises a bacteriocin cell wall-binding domain (CBD) and a second portion, which comprises an enzymatic active domain (EAD) selected from the lytic domain of a bacteriophage lysin, a bacteriocin and a bacterial autolysin. Provided are such chimeric polypeptides and variants and fragments thereof, nucleic acids encoding the same, vectors carrying such nucleic acids and host cells transformed or transfected with such vectors. The chimeric polypeptides of the present invention are useful for the reduction of certain bacterial populations, including methods and compositions for the treatment of various bacterial infections. For example, chimeric polypeptides of the present invention have been shown to effectively kill various bacteria, including methicillin-resistant(MRSA), as well as other human pathogens. Thus, provided are compositions comprising chimeric polypeptides according to the present invention, variants or fragments thereof, and the use of such compositions in prophylaxis or therapy of bacterial diseases, bacterial infections or bacterial colonizations.

The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harboring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.

The present invention relates to a novel tail spike protein (TSP) encoded by the novelbacteriophage designated ProCC P825 and uses of the novel TSP for identifying, detecting and monitoring of

Growing community of inventors

Weilheim, Germany

Sonja Molinaro